FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_2019poolD_UB_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_2019poolD_UB_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490530
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19030.3879477300063197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCG207.896636E-570.049071
ACGCCCG650.070.0490651
CGGGGCG455.456968E-1270.0490651
GAGCCCG301.01810656E-770.0490651
GCGCCGC1450.070.013362
GTAGCGC900.070.013362
AGCGCGC600.070.013362
CGCGCTG1250.069.9990844
CCGCGCT3300.069.999083
GGCGCTA14400.069.512984
CCGCTAG7600.069.078045
CAGCGCT6550.068.93043
CGCTATC8900.068.819336
CCAGCGC2900.068.806232
GCGCTAG7450.068.58975
CGCTCCC2450.068.570536
CCGCTAT16900.068.54945
GCGCTAT16750.068.5364155
CCCGCTA11500.068.4773564
GAGCGCT6500.068.383723
Produced by FastQC (version 0.11.5)