FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_2019poolD_Input_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_2019poolD_Input_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23540817
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG928290.39433210835460814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA675700.068.931184
GCGCTAT831750.068.9000245
GGGCGCT369500.068.620873
GGCGCTC445000.068.564064
GCGCTAG379950.068.5378345
GCGCTCT555850.068.245735
GCCCGCT243800.068.20933
GCGCTCA408050.068.1926655
CGGCGCT350800.068.167993
AGCGCTA524400.068.054464
CGCGCTC220450.068.034664
CAGCGCT363700.067.886663
CGCGCTA335800.067.845814
CGCTATA854950.067.751676
CGCTATT981700.067.64616
CCCCGCT365500.067.6002043
CGCTAGA565550.067.597976
GTGCGCT236800.067.579783
TGGCGCT414850.067.548653
GCCGCTA461750.067.49444
Produced by FastQC (version 0.11.5)