FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_2019poolD_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_2019poolD_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29902
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1220.4079994649187345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTATA207.8032026E-570.000018
ACCGCTA353.5506673E-970.000014
GCTATTT207.8032026E-570.000017
GCTATAC207.8032026E-570.000017
TTGTCCT207.8032026E-570.000013
GGTCCGC207.8032026E-570.000012
CGCGCTA353.5506673E-970.000014
GCGCCGC207.8032026E-570.000012
CGCTGTC353.5506673E-970.000016
CGCTGTA353.5506673E-970.000016
CGCTGGT207.8032026E-570.000016
AGGCGCT353.5506673E-970.000013
GTGGCGC207.8032026E-570.000012
CCGCTAG207.8032026E-570.000015
CCGCTAC207.8032026E-570.000015
CCTCGCT401.2732926E-1070.000013
CTGCGCT207.8032026E-570.000013
GCTCTGA207.8032026E-570.000017
CGCTCTA353.5506673E-970.000016
CCTCCTA207.8032026E-570.000014
Produced by FastQC (version 0.11.5)