FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColLHY_F1b_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColLHY_F1b_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17275471
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG386040.22346134585852973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA13350.025.16809559
GGTCGCC14700.024.28599256
TGGTCGC18500.018.72994255
GTCGCCG20900.017.24899957
CTCGGTG22750.017.07662250
GCCGTAT22900.016.35342460
TCGCCGT21050.016.29424958
GTGGTCG25000.014.9801754
CCGTATC27850.012.81844561
TCGGTGG31900.012.61769851
GGTGGTC29850.012.546205553
GTGTGCC30850.012.3660730
GTGCCTC33450.010.46319432
CGGTGGT40250.09.91315652
CGTATCA39950.09.19883662
GCCTCTA39400.09.14982334
AGTGTGC42500.09.05866429
TCTCGGT44350.08.83862749
GATCTCG45950.08.53086247
ATCTCGG48700.08.48034448
Produced by FastQC (version 0.11.5)