FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColLHY_F1a_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColLHY_F1a_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7792459
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146840.18843859172053393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC5500.019.72726856
CGCCGTA5750.017.65194359
GCCGTAT7200.015.55535360
AGCGGGC1500.005939811513.99981831
TGGTCGC7600.013.81578755
CTCGGTG9350.011.97845350
TCGGTGG11400.011.97368151
GTCGCCG10300.011.2135957
CTGCGCA3351.0569516E-410.44775934
CGGCCTG3156.9655775E-49.99986921
GCGTCGT13400.09.92524312
AGCGTCG12450.09.8392311
GTAGGCT12150.09.50604832
GGTGGTC12750.09.33333253
GTGGTCG12000.09.33333254
AGGGGGG10950.09.2732721
TCGCCGT12300.09.10557258
TGCGCAG3854.2868007E-49.09096635
CGTCGTG14750.09.01683113
ACGCCCC3500.00182538468.99988332
Produced by FastQC (version 0.11.5)