FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColCAMTA1_Col_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColCAMTA1_Col_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988826
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG950.007210420418.42082621
GACCGTA950.007210420418.42082643
ACCCGAC1000.00969012917.49978470
CACTCCC1350.002946457415.55536542
CTTGTCG3751.797107E-712.141781
AGTGAGT3000.0052223189.33321930
ATATGAG12200.08.6125491
TATGAGT12000.08.166982
CTATACA6059.035575E-68.1048121
ATGATAC5653.4058954E-58.05299911
ATATACG5754.2031526E-57.9185521
ATCCTTC4905.3001096E-47.86261221
ATTAGGG4050.00676543547.7831921
CATTAGA4550.00222573437.6976631
CTTTACG4600.00247773747.6139921
AAGGACA5209.998804E-47.4091
AGGATGA4750.00339207457.3735511
CTATTTC7201.1532922E-57.2967431
CTATGAT5600.00219194036.8797861
TGCGGTT9201.0482399E-66.84774264
Produced by FastQC (version 0.11.5)