FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColCAMTA1_C24_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColCAMTA1_C24_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714162
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59700.34827513385549325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3101.4966645E-814.68162251
TCAACGG4500.00201237397.77773638
TGTAGGG6552.6324962E-57.480875569
CGTGTAG7702.1952823E-46.363601767
GTCGTGT8409.983324E-56.249965765
GAGCGTC10104.8581423E-66.237590370
ACGGTTT9457.777071E-55.9276231
CGTCGTG9102.981981E-45.769199464
GTGTAGG7900.00205494535.759462468
GCGTCGT9304.0035896E-45.645130663
TCGTGTA9100.00185597485.38458666
TCGGAAG18003.1008312E-85.24997268
AGCGTCG9350.0026084085.240613562
GATCGGA22202.237357E-105.202674470
AGAGCGT12803.8330283E-55.195284469
Produced by FastQC (version 0.11.5)