FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_CAMTA1_2019poolD_UB_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_CAMTA1_2019poolD_UB_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104746
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91020.2931640784785616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA13650.053.0780834
GGAGTAT30650.052.187115
GGTCCTA14900.051.9138834
CGAGTAT28650.051.432195
GTCCTAT23050.049.8058625
GGGAGTA21000.049.667754
GTCCTAG13900.049.101795
AGGTCCT13850.049.027923
GAGTATT45850.048.2437746
CGGAGTA14350.048.049834
AGTCCTA24850.047.8883744
ATCCTAT34950.047.6691365
CTGAGTA22600.047.2355464
CATCCTA20400.047.1824044
TCCTATA34500.046.8690836
CCGAGTA20000.046.726024
CCGGAGT8500.046.531933
CGCGAGT8500.046.120143
GGAGTCT22800.045.7466135
CCCGAGT12000.045.5024573
Produced by FastQC (version 0.11.5)