FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_CAMTA1_2019poolD_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_CAMTA1_2019poolD_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439195
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCG950.007211817418.42089710
CGCCGTA3250.018.30753965
GGTCGCC3251.0004442E-1016.15371162
CCGTATC3351.5825208E-1015.6715167
GAGACTC4301.8189894E-1214.6510396
TCGCCTG4550.014.61526254
GCCTGTG4900.014.28559456
TGGTCGC3454.0017767E-914.20277861
GCCGTAT3758.60382E-1013.99988266
CGCCTGT4505.456968E-1213.99988155
TGTCGGG1550.0073760213.54827228
CTCGGTG5450.013.48612468
TCGCACC1600.00909803213.12488958
GTAGATC7300.012.46564862
CTTCGCC5357.2759576E-1212.42980152
GAACTCC3407.323288E-712.3528378
CCGAGGC2000.002782175312.24989727
GTGGTCG4003.1783202E-812.24989760
TCGCCGT4601.3842509E-912.17381164
CCTGTGT5501.2732926E-1112.09080757
Produced by FastQC (version 0.11.5)