FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_C24CAMTA1_F1b_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_C24CAMTA1_F1b_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11766171
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG832670.707681368900724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG337850.065.347687
TCCACTG661250.064.536786
CACTAGT321350.064.248428
CCACTGT600350.063.9768457
ACTAGTG320250.063.9226659
CTAGTGG319250.063.8817110
TCCACTA396400.063.1475146
CACTGTA571900.062.8571978
ATCCACT479350.062.801725
GTCCACT259450.061.785585
TCCACTC272900.061.765426
CTCCACT313750.061.6010325
ACTGTAG571550.061.572999
CCACTCT245650.060.6668327
TTCCACT380650.058.709775
CTGTAGA591400.057.44091810
TAGTGGA356800.057.24701711
CGTCCAC68000.055.22914
AGTCCAC102500.054.6011474
AATCCAC211450.053.713594
Produced by FastQC (version 0.11.5)