FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_C24CAMTA1_F1a_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_C24CAMTA1_F1a_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12508268
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG411660.3291103132743878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT154800.056.0040327
TCCACTG180000.055.1050726
CCACTAG108400.054.3722157
CACTGTA149200.054.1416138
ACTGTAG148250.053.260499
CTAGTGG102950.051.84468510
CACTAGT106000.051.2118158
ACTAGTG105900.050.6318249
CTGTAGA158000.048.4675410
GTCCACT83650.047.6578185
ATCCACT160000.047.4477845
TCCACTA143450.046.8939746
TCCACTC96200.043.949726
CTCCACT108950.043.56215
CCACTCT88800.041.18777
TAGTGGA131900.040.6247911
TTCCACT144500.037.9557465
GTAGAAG191050.037.18859512
TGTAGAA201750.035.92752511
CGTCCAC30950.033.1349874
Produced by FastQC (version 0.11.5)