FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_C24CAMTA1_Col_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_C24CAMTA1_Col_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739273
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT1600.00910177413.1248550
CGGGGCG3305.1081224E-712.72712710
GCGGCCT3407.332819E-712.35296439
CGGCCTA3853.276904E-610.90911240
GGGGCGT4401.4349771E-610.3407911
CCTCCCG3401.2277006E-410.29459
GGCGGCC4106.950717E-610.24392138
CCACGGC3208.0470025E-49.84363742
CCGCACA3100.00684584269.03215569
ATTGCGG6301.8454011E-78.888906535
TCGGGGC5201.20361565E-58.74999
GCCCGGA4451.8942676E-48.65170255
GCCTCCC4057.10302E-48.64187658
GGCGTGG4905.7134683E-58.5713313
ACCGACG5754.621079E-68.52164220
GGTGGCG5351.7221459E-58.50468935
CCCGGAG5401.9358884E-58.42594156
CCGTCAG4653.0471326E-48.27947518
TCCCGTA4753.8319337E-48.1051761
AAGGCGG4400.00161414837.95445426
Produced by FastQC (version 0.11.5)