FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_C24CAMTA1_C24_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_C24CAMTA1_C24_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716390
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG407390.5279541339927091No Hit
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA195150.2529032358395571Illumina Single End PCR Primer 1 (96% over 33bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA119530.15490404191597368Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA84500.067.144564
GGGCGCT37000.066.503393
GGCGCTC46400.066.30644
GCGCTAT110850.065.107215
GCGCTAG51450.064.831135
CCGCTAT99450.064.264645
GCCGCTA61800.063.54614
CCGCTAG50150.063.5106475
CCCGCTA50100.063.365684
TGCGCTA47500.062.855024
CGCTATA110950.062.7120636
CGCTAGA80100.062.221366
CGCTATT127400.062.0595786
GCGCTAA66850.061.7288935
CGCTATG56350.061.614056
TGGCGCT49350.061.4924963
AGGCGCT50650.061.296313
CGCTAAT84850.061.295566
GCCCGCT22100.061.2927133
CGGCGCT42700.061.232633
Produced by FastQC (version 0.11.5)