FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37861375
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG813570.2148812609156429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT38450.059.8895263
GCGCTAT36100.054.0118145
GCGCTAG23150.052.8297845
GGTCCTA55750.050.267964
CGCTAGA37300.048.4332356
GAGCGCT19000.048.368653
GGGTCCT50750.047.9908563
GCGCTCA21250.047.1937755
GTCCTAT64700.046.2847945
GTGCGCT18500.045.3316273
AGCGCTA23800.044.633244
CCGCTAG19700.043.2798165
CACTGTA71400.042.6265348
TGCGCTA20850.041.730644
GCGCTAA32850.041.4849935
CGCTATA49300.041.4639556
CGCTAGT24050.041.40876
AGTTCGG32050.040.8849337
CGGTCCT24150.040.6584663
TGGGCGC12100.040.153692
Produced by FastQC (version 0.11.5)