FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_SPL9_2019poolG_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_SPL9_2019poolG_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11643040
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT301400.067.8513953
GGTCCTA327500.066.9111564
GTCCTAT422000.065.064885
CGGTCCT135650.064.968383
GGGGAGT188250.064.756683
GCGGTCC69150.064.332822
GGGGTCC116200.064.278762
GGTGTCC149950.064.0963442
GGGAGTA213150.064.0227054
GCGAGTA123050.063.9412044
GGTCCTC175600.063.043574
GTGTCCT333900.063.0395553
AGTTCGG124850.062.8228387
GGCGAGT92550.062.7009663
TGTCCTA328550.062.5427174
CGGGAGT95300.062.360693
GTCCTAG187350.061.9853135
CGTCCTA118450.061.5784534
AGGGTCC81000.061.5324442
GGAGTTC273700.061.329655
Produced by FastQC (version 0.11.5)