FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_Col_UB_g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_Col_UB_g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723520
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG556.6959037E-638.1817056
TCGGACT1151.4588295E-930.4346925
CGGACTC901.9279502E-423.3332656
GGACTGT1301.056972E-418.8460987
TCAGGAC1000.00968717117.49994943
ACTGTAG1901.1330198E-414.7367979
GACTGTA1550.007371090413.5483478
CCCAGAA1600.00909198313.12496218
TACGACA1600.00909198313.12496233
AACGCAA2254.7451849E-412.44440762
CTCGGAC2050.00334443611.9511844
AGTATAC2300.0078841210.65214323
GTAGAAG2650.001868427610.56600712
CTGTAGA2350.00924706710.42550210
ATAAGCT3150.00779285388.8888633
Produced by FastQC (version 0.11.5)