FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_Col_UB_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_Col_UB_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53437
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTA455.456968E-1270.000018
CGGACTG500.070.000016
TGCTTCC150.002213273770.023
ACGGACT207.859699E-570.05
TCCCGAT150.002213273770.051
GACGACT150.002213273770.024
CATCCCG150.002213273770.049
ACTGTAG550.063.6363689
CTGTAGA601.8189894E-1258.33333210
GGACTGT601.8189894E-1258.3333327
ATCCTTA200.00691597252.544
AAGGTAA200.00691597252.552
TATGATA200.00691597252.561
TCGGACT200.00691597252.55
TGACCAA200.00691597252.524
ATCCCGA200.00691597252.550
CCGCATC200.00691597252.546
GTAGAAG751.4551915E-1146.66666812
CTACAGT451.6555659E-646.66666824
CAGTCCG451.6555659E-646.66666827
Produced by FastQC (version 0.11.5)