FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_Col_UB_g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_Col_UB_g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886587
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4150.063.2523966
ACTGTAG14800.057.7021459
CCACTGT12500.056.5594527
TCCACTG13500.056.2587176
CGCGGAC509.822543E-1055.999464
CCACTAG7050.055.6023037
CTGTAGA14900.053.79142810
GCGGACT1900.053.4205325
CACTGTA12300.053.210878
GGACTGT4700.052.871837
CTAGTGG7250.051.65467510
CGGACTA2150.050.4646266
ATCCACT9550.048.742995
ACTAGTG7700.048.6358959
CACTAGT7800.048.461078
TCCACTA8850.048.248126
CCGGACT2300.047.1734545
GGGCGCT451.6854665E-646.6662143
GACTGTA4900.045.713858
TGTAGAA17150.044.08120711
Produced by FastQC (version 0.11.5)