FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_Col_B_g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_Col_B_g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426791
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGC305.87542E-446.6663568
CTGGTGA1107.466927E-419.09078215
TGCTTAC1500.0059274313.99990654
AGTTTAT3950.0054274767.974636
GAATATG9000.0094371255.05789231
Produced by FastQC (version 0.11.5)