FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_Col_B_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_Col_B_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27199
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA150.002201516670.05
GGGTCCT150.002201516670.03
GGATCTT150.002201516670.032
GGTTCCT150.002201516670.03
GTCCTCT150.002201516670.05
AGAGTCT150.002201516670.05
GATCCTT150.002201516670.04
GGGATCT150.002201516670.031
AGTCCTC150.002201516670.04
GTCCTAT455.456968E-1269.999995
TCCTCTA200.00687951752.56
TCTAGAC200.00687951752.540
AACGAGT200.00687951752.53
GTTCCTA200.00687951752.54
GGTCCTA407.214894E-752.54
TAGTCCT200.00687951752.53
TCAAAGG200.00687951752.526
AGTCCTA200.00687951752.54
TGAGTGT305.7780097E-446.6666645
GTATCCT350.001234241940.03
Produced by FastQC (version 0.11.5)