FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_2019poolF_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_2019poolF_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519456
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGGGAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT93160.36976236139865115TruSeq Adapter, Index 10 (97% over 35bp)
GGCGGGAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT80550.3197118743093747TruSeq Adapter, Index 10 (97% over 35bp)
GAGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT62430.24779158675523605TruSeq Adapter, Index 3 (97% over 36bp)
GGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT49470.1963519108886998TruSeq Adapter, Index 3 (97% over 36bp)
GGGTGGAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT35760.14193540192803525TruSeq Adapter, Index 10 (97% over 35bp)
GGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT32340.1283610430188104TruSeq Adapter, Index 3 (97% over 36bp)
ACGTCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG28760.11415162638283821TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGT107450.068.6314543
AGTTCGG116600.068.6187447
GGAGTTC231250.068.395215
GAGTTCG118900.068.086166
GGGAGTA94150.067.843414
GGGAGTC53300.067.832564
CGCGAGT21700.067.741483
GCGAGTA56850.067.721624
CGGGAGT57950.067.5836643
GGGAGTT88350.067.5830154
GAGTCTC140350.067.45596
GTTCGGA118000.067.270738
TTCGGAA117250.066.8950659
GGCGAGT48350.066.8868263
GCGAGTC33500.066.865224
GGGGCGA11000.066.840281
CGAGTGC27700.066.5885
GGAGTAT101900.066.5648045
CGAGTAT92800.066.416575
GAGTATC79600.066.2621156
Produced by FastQC (version 0.11.5)