FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_2019poolE_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_2019poolE_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773462
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT421050.068.8189543
GGTCCTA453550.068.502264
GTCCTAT436550.068.259575
GGGGTCC158800.067.818232
CGGTCCT163750.067.648213
GCGGTCC86400.066.962022
GTCCTAA319850.066.782225
GGTCCTC249100.066.767724
GTCCTAG221000.066.705245
GTGTCCT259800.066.67183
GGCTCCT188800.066.477123
CGTCCTA121000.066.3258064
GAGTCCT258650.066.223993
TGTCCTA281850.066.087714
AGTCCTA270800.066.044424
AGGTCCT216900.065.8522953
GGTTCCT284300.065.776713
GCGCTCC31300.065.7510152
GGTCCTT193550.065.713654
GCGTCCT98300.065.6911243
Produced by FastQC (version 0.11.5)