FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_2019poolD_UB_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_2019poolD_UB_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490530
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCG353.6834535E-970.056111
GGCACGC950.070.013262
GCGCCGC2000.070.013252
CGGGCGC3150.070.013252
GCGCTGT4450.069.9989855
CAGGGCC150.002224270769.9989845
CGCTCAT10300.069.659186
GGGCGCT19700.069.288343
GCCGCTA9800.069.28474
CGCTAGT8650.069.189756
GGTGCGC3900.069.1156542
CCGCTAT14100.069.006095
GGGGCCG2900.068.848241
GCGCTCA9000.068.832335
GTCGCTA11300.068.760064
GCGCTAG8100.068.7027055
ACGGCGC2550.068.640452
GTGCGCT7600.068.6174243
GCGCGCT4600.068.477263
GAGCGCT6800.068.454893
Produced by FastQC (version 0.11.5)