FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_2019poolD_Input_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_2019poolD_Input_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23540817
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT923750.069.434383
GGCGCTA954950.069.3172154
GCGCTAT906500.069.118625
GCGCTAG411750.068.7748955
GGCCGCT515250.068.579253
GCGCTCA411500.068.578535
CGGGCGC162200.068.533362
AGCGCTA453050.068.4924854
GCGCTCT552350.068.459165
GGGGCGC343150.068.429952
GCCCGCT287550.068.307073
CGGCGCT298800.068.195073
GAGCGCT373350.068.189653
AGGGCGC248850.068.130082
GGTGCGC164500.068.107072
GGGCCGC286050.068.0552142
GCCGCTA572950.068.007514
CGCTAGA592100.067.953696
CGCTATA899050.067.920096
GTGCGCT375300.067.881983
Produced by FastQC (version 0.11.5)