FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColTGA5_2019poolD_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColTGA5_2019poolD_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29902
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTG207.8032026E-570.000014
GAGGCGC353.5506673E-970.000012
CGCTGAA207.8032026E-570.000016
GTGTCCT353.5506673E-970.000013
ACGCTCT207.8032026E-570.000015
TTCGCTC353.5506673E-970.000014
GTCGCTT207.8032026E-570.000014
GTCGCTC207.8032026E-570.000014
GTCGCTA401.2732926E-1070.000014
GGCGCTA1050.070.000014
TCCTACC353.5506673E-970.000016
CCTAATA207.8032026E-570.000017
TGCCGCT401.2732926E-1070.000013
GTCCGCT207.8032026E-570.000013
CCGCTAC353.5506673E-970.000015
CCGCTAA401.2732926E-1070.000015
CGCTTGT207.8032026E-570.000016
ACCGCTA353.5506673E-970.000014
GCGGCGC207.8032026E-570.000012
CGCTCCA207.8032026E-570.000016
Produced by FastQC (version 0.11.5)