FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColLHY_F1b_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColLHY_F1b_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17275471
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25000.024.07962250
ATGCCGT30150.021.12736549
GCCGTCT23050.020.49859651
TATGCCG34600.019.6239748
GTATGCC35850.019.13520847
CGTATGC35500.018.8309146
CGGGGTG7400.017.50028453
CAGTCAC82650.015.83780827
CTGCGTG13800.015.47126659
TCCAGTC87550.015.11130325
GCGGGGT7200.015.06920952
CCAGTCA87500.014.9199426
GCGTGAA23800.014.55859661
TGCCGGC6050.014.46258450
CTCCAGT93850.014.0594524
CATTCAG97150.013.61790433
ACTCCAG97350.013.58992723
CGTCTGA97550.013.45446816
ACGTCTG98800.013.24881915
GAACTCC101100.012.94737421
Produced by FastQC (version 0.11.5)