FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColLHY_F1a_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColLHY_F1a_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7792459
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7000.012.99980450
ACGTCTG18900.012.592401515
GCACACG18050.012.40978311
CGCCCGC2150.00478123811.39517532
CAGTCAC19900.011.08037627
GTATGCC9850.011.015202547
ACGACGC6805.456968E-1210.808665
CGTCTGA21250.010.7057216
CACGTCT21450.010.605914
GGCCCGC2650.00187215310.56587710
TCCAGTC22600.010.53094925
ACACGTC21550.010.39427413
CGACGCT6452.382876E-1010.309926
TATGCCG9550.010.26162448
CACACGT22750.09.99984812
CTCCAGT24400.09.8973924
ATGCCGT9600.09.843649
CCAGTCA23550.09.660274526
GACGCTC6603.8635335E-99.5453097
GCGGCGT2950.00455235979.49150330
Produced by FastQC (version 0.11.5)