FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColCAMTA1_F1a_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColCAMTA1_F1a_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744683
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2207.27905E-817.49993751
TATGCCG2451.32968125E-817.14279448
CGCTCAT2903.4197E-1016.8964934
TGCCGTC2552.1784217E-816.47052650
GTATGCC3057.021299E-1016.06551647
CCGCTCA3201.3842509E-915.31244433
ATGCCGT2951.3029057E-714.23723649
CGTATGC3102.386314E-713.54833846
ACCGCTC3558.893403E-812.81685432
ACTCCAG4802.7066562E-911.66662323
TCCAGTC4451.3958015E-711.01119525
CTCGTAT4259.1765287E-710.70584244
GTCACCG4602.2051609E-710.65213529
CACCGCT4602.2051609E-710.65213531
ACGCTCT4055.9805807E-610.3730081
TCACCGC5108.213101E-810.29407930
CAGTCAC5159.473115E-810.19413827
CCAGTCA5201.09099346E-710.09611626
AGTCACC4804.358173E-69.47913128
GCACACG5251.3467779E-69.333311
Produced by FastQC (version 0.11.5)