FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColCAMTA1_Col_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColCAMTA1_Col_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988826
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACA450.00430831131.11090717
GGTCGTC900.00527655219.44431765
GACAGGC950.00721017818.42093342
GGGACAC950.00721017818.42093334
CGCTAAG1150.001007926318.2607528
CGCGGCC1000.00968980617.49988627
GTAGGCC1656.519313E-414.84838856
GCCTGCA1450.004739486614.48266531
GTCACCG1500.00593471613.99990969
AATGCGG1550.00737377713.548320
GACCCCA1600.00909528113.12491521
TGTCCTC2500.001149756411.19992763
CCTGATG2250.006679691410.8932251
CTATGTT5351.4639227E-810.4714581
GAGTACG2350.0092513310.42546514
CTTTACA5452.1459346E-79.6368631
CGGGTTG3350.00122239.40292461
CAACCCA3100.0068381299.032210
TTGGACC3150.0077972918.88883127
TGGTACA3600.0023539248.74994320
Produced by FastQC (version 0.11.5)