FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_ColCAMTA1_C24_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_ColCAMTA1_C24_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714162
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1751.2626151E-720.0056931
GTATGCC2350.009256015510.42545844
CGCTCAT2750.00254514810.181746531
GATACTC5200.0082335246.730721532
CAGTCAC5850.00349766416.581149624
GCAACTC6500.00147586756.461492538
CAGATCG7358.6512393E-46.19043268
ACGTCTG8053.897208E-46.086913667
CGTCTGA8457.2544755E-45.798775768
GCACACG9656.587528E-45.440376363
TCTGAAC9707.060841E-45.41233370
GAGCACA13653.5593093E-65.384577370
GTCTGAA10453.3057228E-45.358813369
CACGTCT10906.0029875E-45.137578566
TCGGAAG18505.824222E-85.108072370
GATCGGA22955.620677E-105.03264470