FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_CAMTA1_2019poolD_UB_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_CAMTA1_2019poolD_UB_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104746
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT26450.062.986133
GGTCCTA30400.060.4434664
GGGGAGT20250.057.3821373
GCGAGTA13300.055.7889064
GTCCTAT31500.055.777215
GGGAGTA24900.054.53764
GGGCGCT2700.054.4438933
CGGTCCT11600.053.404633
CGAGTAT27750.052.720195
GGAGTAT32500.052.5533145
GGGGTCC11650.052.2741472
GGCGAGT13000.052.230243
GGTCCTC17750.050.8727264
GGCTCCT15150.049.900483
GGTGTCC9750.049.896932
GCGGTCC6150.049.5116922
GTGTCCT20300.048.275373
GAGTATT46250.048.2049186
CGGAGTA9400.048.031434
AGGTCCT16550.047.794083
Produced by FastQC (version 0.11.5)