FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_CAMTA1_2019poolD_Input_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_CAMTA1_2019poolD_Input_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111559
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3153.6379788E-1217.77765750
CCGCTCA3658.8439265E-913.42456633
GTATGCC3558.897223E-812.81681447
ATGCCGT4153.916284E-912.650516549
TATGCCG3601.0679105E-712.638803548
ACCGCTC4409.336873E-911.93173832
CGCTCAT4551.53359E-811.538383534
GCCGTCT3358.367637E-611.49245951
TCCAGTC6502.3646862E-1110.76915725
GAGCGGT2350.00925718910.4254615
CTCGTAT5301.4414582E-79.90559344
ACTCCAG6857.1122486E-109.70796423
CGTATGC4703.3341257E-69.68078546
GTCACCG6201.4113539E-89.59670929
CAGTCAC6251.609078E-89.51993627
GCACACG7302.244633E-99.10952811
TCACCGC6151.2741657E-79.1056330
TCGTATG6553.4515324E-89.08390845
CCAGTCA7101.34823495E-88.87317926
GAACTCC7503.6525307E-98.86660721