FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_CAMTA1_2019poolD_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_CAMTA1_2019poolD_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439195
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT2400.030.6257613
GGCGCTA703.4908582E-530.0007444
GGAGTTC3350.020.896045
GCGCTCT1107.477127E-419.0913835
GGTACCG950.007211877518.4208753
CGGTACC1150.001008253218.26068952
GCGCTAT1200.00134106817.5004355
GCGCAGC1000.00969207717.49982642
AGGGTCC1906.1224746E-616.579362
GCGGCCA1300.002291327316.15368549
ACGGTAC1300.002291327316.15368551
GGTCCTA2401.9607978E-716.0420654
GGGGGAG1755.6212462E-516.0003972
GAGTCTC5450.014.77100856
GGGGAGT2656.03035E-714.5286633
GTCCTAT4351.8189894E-1214.4831175
ACCGCTC6050.014.462666551
CGCTAGA1500.005935217714.0003476
ATGCCGT5550.013.87373568
AGTCCTG1800.001259305913.6114484
Produced by FastQC (version 0.11.5)