FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_C24CAMTA1_F1b_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_C24CAMTA1_F1b_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11766171
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG342100.065.20097
CCACTGT670250.065.0070347
TCCACTG728050.064.913296
CACTGTA629200.064.425478
ACTAGTG326050.064.052289
CACTAGT329400.064.0498
CTAGTGG326000.063.94400810
ACTGTAG617900.063.819429
TCCACTA397650.063.0373466
ATCCACT449000.062.4694335
GTCCACT272750.061.7611965
CTGTAGA596600.061.3577810
CTCCACT329900.060.24965
TTCCACT378600.058.4253165
TAGTGGA366950.056.8367511
CCACTCT178100.056.5967257
TCCACTC205450.056.4390756
CGTCCAC75250.056.139094
TGTAGAA584500.056.0831511
AGTCCAC105600.052.996744
Produced by FastQC (version 0.11.5)