FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_C24CAMTA1_F1a_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_C24CAMTA1_F1a_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12508268
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT180600.056.762637
TCCACTG197250.056.3896646
CACTGTA171800.055.697578
ACTGTAG165400.055.673219
CCACTAG111000.053.1297577
CTAGTGG105850.051.6477110
CACTAGT107200.051.5523228
CTGTAGA168550.050.45897710
ACTAGTG109950.049.8172769
TCCACTA140250.047.564476
ATCCACT149750.047.0244565
GTCCACT85500.046.870765
CTCCACT116300.043.335665
TAGTGGA135250.040.70543311
TTCCACT146300.037.9660345
TGTAGAA201950.037.72901511
TCCACTC81100.037.373156
GTAGAAG202600.036.4850912
CCACTCT72650.035.6979687
CGTCCAC29500.032.982644
Produced by FastQC (version 0.11.5)