FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_C24CAMTA1_Col_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_C24CAMTA1_Col_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739273
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3951.8644641E-913.2963241
GGGAGCG2305.7193136E-412.17393946
CTGGCGC2050.00334912111.95108551
GGCCACC2550.001358226610.980268560
GCCGGAC2350.00925973410.42541411
GCGACCG4552.2049335E-69.999886538
GTGCGGG4005.9293758E-59.6250226
CGCTACC3000.00522741639.33335359
TGCGGGC3803.7602108E-49.21054627
CCCGGGG3100.0068450789.03227756
TAGCCTG3100.00684582959.03215717
GCGGCCT3150.00780604278.88878839
ATCGGGC3600.00235690948.75001956
GAGCGAC4552.4096557E-48.46144336
GCGCCTA3800.00384451548.2893863
TGTGCGG4000.0060981727.87501625
GGACGGG4000.00609890377.87491136
AGCGGGC4450.0018042087.865079419
GCCTCGA4500.0020135887.777794456
ATGACCG4100.00760882677.682840334
Produced by FastQC (version 0.11.5)