FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n01_C24CAMTA1_C24_B_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n01_C24CAMTA1_C24_B_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716390
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC219110.28395402513351453TruSeq Adapter, Index 7 (97% over 38bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC142650.1848662392647339TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA162950.068.667644
GCGCTAG130400.068.308395
GGGCGCT108800.068.262213
CGCTAGA274850.068.0255366
CCGCTAG113100.067.337995
GGCGCTC66100.066.981194
GCGCTAT109100.066.566725
GAGCGCT104850.066.5277253
GCCGCTA128400.066.4834
AGCGCTA88400.066.396414
GCGCTCA63900.066.1104665
CGGCGCT78100.065.9660643
GGCCGCT63350.065.689973
AGCGCTC74950.065.189494
GGAGCGC46300.065.163472
GCCCGCT32100.064.9837953
CGGGCGC21550.064.641842
GCGCGCT51350.064.614753
CAGCGCT45100.064.4893953
GCGCTCC34750.064.056935
Produced by FastQC (version 0.11.5)