FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n02_MCF7_DMSO_22_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n02_MCF7_DMSO_22_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACCCAAGTGTGCCTTGTGGCACCAAAGGTCTACAGGGACCCAGT33.7037037037037033No Hit
TAGCCCTCTCGTCAATGTCTCGGATCGATCCATCTTGAGTTGGTTTTTGTA22.4691358024691357No Hit
AAACTATAGATATCCACATAAATATAAATTGAAAACTTGTCGAACCGTGAA11.2345679012345678No Hit
AACACTTCCTAAGATTTACTTTCTCTAACATGACAATCACACTTACGAATG11.2345679012345678No Hit
CACCCTAACAAATTTAAAAAATGAGAAATCATACAACTTCTACTCTCAGAT11.2345679012345678No Hit
CTCCATTATCATGCAGCAAGTATTGTACTTTCTACATAAACTGAGCAGTGC11.2345679012345678No Hit
TCATAGCAGCACTACCTATGTCACTCTTAATCCGTTTCCTGCCATAATAAT11.2345679012345678No Hit
TTCAAGACTCGAACAAATAACTCCAATATGCCCTTTACTGCATTCGGTGGC11.2345679012345678No Hit
TGTTTTTTTTTTTTTTTTTTTTTGACGGAGTATTGAGCGGTCACCAGGCTG11.2345679012345678No Hit
CGTGTTCGATATACTGATCTGAAAAGAGTTTTGATACAAATATATGTAGTA11.2345679012345678No Hit
GAGTTTAGTTATATCGATCATATCACAATAAATAATCAGAACTAAACAACA11.2345679012345678No Hit
CCAAAGGGGGCATTGTGAAATATCTCTGCCTGATCGATCCTTTACACAGAG11.2345679012345678No Hit
TTCCTCCTTGGGACTGAATACAAGAAGTGTAGAGGCAATCAGGTCGCGAGT11.2345679012345678No Hit
CCCTCAAAGAAAGATAGCATTTCCCAAACCTGACTCCTATTAAGTGAAAAC11.2345679012345678No Hit
AACATATATCATTATTCAAAATACATTACCCAACCTCACACAAGCAAACCA11.2345679012345678No Hit
ACCTTCAATAAGAGAAAGGAAGTAGAGTGGAAAGAGCAGGAGAATTGAGTA11.2345679012345678No Hit
CCTTCTGGTAACAGTTCCTTTGTGCCTTTCATCTACATTTAATTGACTGAT11.2345679012345678No Hit
TAAAAATAATTCAAATTATAATCATATATCACATAAAATAAGTACATAAAT11.2345679012345678No Hit
GAGGAGTAAGGGAAGGGCCTGTGATCTCTTCTGTGGCCATTGCTTCTTTGG11.2345679012345678No Hit
AACTCTAGAAGAACAAGATCGATCCTTTGACTGTGTTGGGCACACCATCCT11.2345679012345678No Hit
CTGATATGAAGCACTAAGAAAAACATGTCAGTTTTGAAACATCAGTTCTAT11.2345679012345678No Hit
GTGACAGGACACACTGAGGGAATTGGAGACTATCACTGTAGGTGATCGATC11.2345679012345678No Hit
TCCCTAGTGAGGGTGTGTGTTTGGGGGTGGATGATCCTCCCCTTTCACAGT11.2345679012345678No Hit
CAATATGGAGTGGTGGCTTTTAGCCACCTGAGGTTGAATCCTGATGCTACC11.2345679012345678No Hit
CCTGAAGCATGGTGGTCAAATTGACAGGAAACTAAGAATATCTAAAAGTAA11.2345679012345678No Hit
AGCATCCAAATCAAAAAAAAGTAAATTAAATGCAAATTAAGAAAGATTATC11.2345679012345678No Hit
ACACTTTATTCCACAATAACACACAACCTAAGCCTACAAAATTTCAACAAA11.2345679012345678No Hit
AGGAATAGTGGTTTAAGAGGTTATTATCCAGATAAAAAAGGGAGAGGACAA11.2345679012345678No Hit
TACCCTGTGAAAGGTATTACCTAGGTGGTAGAGATATAACAGGGAATAAAA11.2345679012345678No Hit
AGCACTTTAGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGTTTGTTTT11.2345679012345678No Hit
GCCTACATCTACTAAAACTGCTAATCCCAGTCCATCTCAGGAACTGATCGA11.2345679012345678No Hit
AGAAACTTCAAAAATATTTTTATTTTTTTATGACAAATCAGTAATAAAATA11.2345679012345678No Hit
ATAAATTTAATAAACTGGCATCCTTCAACACACAAATAACAAACAAAAACC11.2345679012345678No Hit
TCAGAAATTTTATATAGTGGATAACTCTTCCTTGATCGATCACTTTGGGCA11.2345679012345678No Hit
ATAATCTCTCTGCTCCCGTCTCTGGTGGATCGATCATTTTATTTTAATTGC11.2345679012345678No Hit
ACACTTTGGGGGTGTCAAGGCAGGAGGATCGATCGAGACTATCCTGGCCAA11.2345679012345678No Hit
TGAAAACTTGATTTTTTAAAAATGTATTTTACTTGGATCGATCTGGTGGAG11.2345679012345678No Hit
TGCTGTGGGCATCAGCGTCTGGACAATGGGAATGCAGTGGCACCCAAAAGC11.2345679012345678No Hit
TAACAGTAGAATGCACACACGAAATGAAAAAATTATTAAATAAAACATAGA11.2345679012345678No Hit
TGTGAAAAACTGTTGTGTTGTGCTGTGTTATATTATGGAATGGACCGTGTG11.2345679012345678No Hit
AGCACAAGAACACCAACCGAGAAACCAACCCCCAGAACGAAACAAAGATTA11.2345679012345678No Hit
AAAGTAAAAGATAAACTCATCCAACATACTACCAACAATCATAAAAAAAAA11.2345679012345678No Hit
GGGCCCCAGGATTAAATCCATGCCACACTTGCATGGACACATATGCCAGTT11.2345679012345678No Hit
CTAAACAATAGCCTTAAATAAGGCTCTAAAAATTAACCACAGGAAATAAAG11.2345679012345678No Hit
ATGTATTATATTTTAGGATCATCCTTGTTTTGTTTCTGCTTTAAGTAGAAA11.2345679012345678No Hit
AAAATCTTCATCCTCATCTACTCCTTTTGTCGGTAGGTTTGGTCTTCTCAT11.2345679012345678No Hit
ATAAACACGACAAACTGAACAAACATAAAATTCTACAACCAAAAAAGCAAA11.2345679012345678No Hit
CCCTGTTTTCTACTAAAAATACAAGAACCCAGGCATGGTGTCTCATGCCTG11.2345679012345678No Hit
CTACGGTCCAAACAACAAAACATAGAAGTAAGGATAAAATTTACCAAAAAC11.2345679012345678No Hit
TGATTTTTGATATATATATATTAAGATTTATTTATTTATCATATGTAAGTA11.2345679012345678No Hit
GACAGGTGGCAGAACCAGCAGGTTTACTGAGGGCTGCCAGGCTCCTCCCTT11.2345679012345678No Hit
GTAAGTACCCTTGATATGACGTGATGGAAATGGCATTTCACCTCTGCGGTC11.2345679012345678No Hit
ATAAACAACAAATCAAACTCAACAACATTCTTAACCTCCTTATCAGATATA11.2345679012345678No Hit
CAATGTCAAAAAGGTAAATCAATAGTTATTTGACAATACATGCTCAGTCCA11.2345679012345678No Hit
TAGCAAATGAACTAGCTACAATATTTTACGATCAACATAACACATAAAAGT11.2345679012345678No Hit
TAAAGAAAATTATGAGTTTTTTTTTTAAATTAGTAATTAATTTTAGAAACA11.2345679012345678No Hit
AACAAATTTGTCCAGTCTGGAGTGCCGAGGCGTGATCTCTGATTACTTCAA11.2345679012345678No Hit
AATTATCGTGGCAATTTATGGCCTACATCTGTAATGGAAGGAAATGCTAAA11.2345679012345678No Hit
CAAAATCCAACACCCATTCATGATAAAAGTCTTGGAAAGATCGATCAGAAC11.2345679012345678No Hit
CGGCTCTCTGTTCTTTATTCCTCACAGCTTAGTAGATCGATCATTTCCATG11.2345679012345678No Hit
ATGAAGTGCAAGACTTGTACAATGAAAACTACAAAACATTGTTGAAAGAAA11.2345679012345678No Hit
TGGGTGACAGGCTAGAGACTTAACGAGGAGAAAGGTAGTTACTAGATATCC11.2345679012345678No Hit
TAAATACCATAAAATGAGATCATCCTACGTGTCAGAATCAATACTGAGACC11.2345679012345678No Hit
TGGCAAGCCTCGTATCAAAATCATGACCTCAAGTGATCGATCCCATGAGCC11.2345679012345678No Hit
AGGTGTAGTTATTGCTATTATGTATCTGACCCGTGACGATCATTTTGACAC11.2345679012345678No Hit
TATAAAAAAATCATCAATTACTCTACAAATACAATATAACACAACTAAAAA11.2345679012345678No Hit
TCCCCGACTTTTGTAATAAGATAGAGATTTATACCCCAACTTCAAAGAAGA11.2345679012345678No Hit
AAAATATAATTATCAAAGTCTCAAGGATACTAAAAGATGTTAAATAAAAAG11.2345679012345678No Hit
TGGAATGGTGTAAAGCTAATGTTCTGGAAGATCGATCATTGCTTTTGAAAT11.2345679012345678No Hit
GTCTCTACTAAAAATACAAAAATTAGCTGGGCATTATGATAGGCACCTGTA11.2345679012345678No Hit
TATACAATATTACTAAACTCCTAAAATTCTAAGTACTCTTCTTCCTGCAAG11.2345679012345678No Hit
TCACATGGAAGTCTACATGTGTGTTTGCACATCTAAAGTACATGTTCCATG11.2345679012345678No Hit
AGAAGCTATGGTTATAAGTTCATGTTGAGGACCTTTGTCCTCCTCTTCATG11.2345679012345678No Hit
CTGGACTACAGTAGCATGATCGATCCCTCAATGTCCATTCGCAATCACTCC11.2345679012345678No Hit
TATCAATTAATCTTGTAATTCAAATTTGGTATATAGCGTTTTAAAAATACA11.2345679012345678No Hit
GGGGGTCTTAAGCGGTAATAAGAGTAATATAGAAATTTTTATTTTTTGAAT11.2345679012345678No Hit
TGGTACAAAAACAGTCACATAGACCAATGGAACAAAATAGTGGACCCAGAA11.2345679012345678No Hit
CTCAGCCTCCCAAGTAGCTGGGATTATAGGCATGTGCCACCACGCCCGGCT11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)