FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n02_MCF7_DMSO_21_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n02_MCF7_DMSO_21_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAAAATATCCCCACCAGAAGCTCACTCTCTCAGTCAAAGGGCACGAGA32.6548672566371683No Hit
TGAGATATTAAAAGACATCCTGTTTCTGCCCCCGTGTCACTTCTGAGATGT21.7699115044247788No Hit
AAGTCTCAGAGGTAATTAATTATACCTGAACTATAATAGCCTTTTAAACAT21.7699115044247788No Hit
TCATTCACATTGCCTGTCCCCAGTGGTGTTTGAATTCGTGACATCCAATAT21.7699115044247788No Hit
AAATAATCACAGAAGGTAGAGAGAAGGAGGAGGGAGAGAGGAGGGGGAGGG21.7699115044247788No Hit
GTGCTCAGTAGGTCCCATATGTTTCTTATATGCCCTGCGGCCTCTGTCAAT21.7699115044247788No Hit
TTGGACCATATGACCCAGCAGATCGATCTTTCCAAGTCCCTGACCATCCAG21.7699115044247788No Hit
AATAAAAAGAAGCCTAAAGCAGCTAAATTAGAACAATAGAAGGTTTATTAG10.8849557522123894No Hit
CCTAAAGCATCCCACTGTATCATTCTCCTACCATCACCTTAAACTGGGTCA10.8849557522123894No Hit
AAATTCAGGATGGTGCTGTCTAGGCCCTAGGGTAAGAGAGTTTCTAAGGAC10.8849557522123894No Hit
TAGAAATCAATTAGAAAAGAAAAATGCTGAATTATAAAACAGCAAACAAAC10.8849557522123894No Hit
ATGCCCAAGTTCTTAAGCTATCCCTTGTCCTGGAGTCCCCTTAAAGTGGCT10.8849557522123894No Hit
GTTCCTGTTGGCTGTGTGTATAGGAGGGACATATTTACCATCACTGTACAT10.8849557522123894No Hit
TGAACAAAGAATTCTCACCTGAGGAATACCGAATGGCAGAGAAGCACCTGA10.8849557522123894No Hit
ACCCCACATCCCACCCCACCCACCACCCCACCACTACCACCCACCACCCCC10.8849557522123894No Hit
CTAATTAATGCAAAGAAACATTTGAAACATGTCATAATATTTGTTGTACAG10.8849557522123894No Hit
TAGAATAATCATGTGTGCTTCCCTTTGATCGATCCAAGAATCCTGCTGGGG10.8849557522123894No Hit
TATATTACAAAACGAACATATATCTCACCTAAATTGAAAATAACATAAAAC10.8849557522123894No Hit
TTATCACTCTTTCTCTGATAAAAGAAATATTATTTGTTTTAAGACTTTTTT10.8849557522123894No Hit
AAGGATTACAGACGTGAGCCACTTCACCCGGCAAGTTTTTAGAATAAAGTA10.8849557522123894No Hit
TAGACAAAGCCTTCTAGAACCAAGCAGAAAGTAGATCGATCTAGGAAAAGT10.8849557522123894No Hit
GACAGCATTTTCAACAAATGGTGCTGGCACAACTGGTTGTTATCATGTAGA10.8849557522123894No Hit
AAACAAACAATCAGAAAAAACTGACAAGTGAATGGACAGTGAGTGGTGGGT10.8849557522123894No Hit
CATTAAAAGCATTCTTTATTTAAAATGGTATTTTACTAACGAATTAACTTG10.8849557522123894No Hit
GGTCTAATCAATAGTCATGCTGGCTATAAGAGCTTTTGACACTAAGATGTC10.8849557522123894No Hit
AAAACCAAAAAAGAAAAAACCAAAAAAAAAAAGCAAAAAAACCAAACAGAA10.8849557522123894No Hit
GTCAACTGTACTTTTTATGTCTGTTGATGATAGAAAAATATGTTAACCTTT10.8849557522123894No Hit
ATTAAACATCGAAATAGATCGATCCACCTGCTTTGGCCTCCCAAAATGCTA10.8849557522123894No Hit
GAGAACTGAGAGTAGGGTTATATCCTTTGCTGTTTGATAGTGAAGTTTACT10.8849557522123894No Hit
AACACACACACAACCAAAAAAAAAACAAACAAAAACAAAAAAGAAAAAGAA10.8849557522123894No Hit
CACAAAACAAAACCCCAGCACAGACCACAACACACACGCCAAAACCGCACA10.8849557522123894No Hit
GAGGAGTGTTCCTCTTTCTCCACATCCTTGCCAGCATCTGCTGTCACCTTA10.8849557522123894No Hit
GTCTAACATTGTTCCATGTCTGCTTTGTTTAACATTGATCGATCCTTTGGG10.8849557522123894No Hit
TGAATGGTTTTGACCAAAATGCCGATAATGATATGGACAGTGAAGTCCAGG10.8849557522123894No Hit
CTTCTAGAAGGCTGAATGTGGTTCTATGTGAGAAAAGGCAGTTAGTTTTCC10.8849557522123894No Hit
TGACTTCAAACTATGCTACAAGGCTACAGTAACCAAAACAGCATGGTACTG10.8849557522123894No Hit
TGATTAAATTACCTCCCAGCAGATCGATCCAGTGACAGTATCAGAAACCAG10.8849557522123894No Hit
TATAAATATTAAAAAGTTGTATCACAAGATCGATCACATATATCTAGCCAG10.8849557522123894No Hit
AACAAAACCTCTCAATCTAACCTGGTCAAAATGCAAAAACCAACAGACACA10.8849557522123894No Hit
ATCACGTGGGATCTAAGATGGAAAAATACTTGTAAACTTTACACTGTAAGA10.8849557522123894No Hit
ATCCAAGTGATGTAACCCTTGTCTAGGATCGATCATTGAAGCGATATGTCA10.8849557522123894No Hit
CCCCCCACCCAAAACAACCCCAAAACCACAGAACAGAGACCCCACACACAC10.8849557522123894No Hit
TCCCTCAAACTAAGGTGCAATGATTAGGTTCTAGCAACACAATGTCCTTCG10.8849557522123894No Hit
TAATGTTGCCTCCTTCTCTCAGAGCTGACTAAGCCCTCAGCAAGCAGCAAA10.8849557522123894No Hit
TCAATACATACCAAACCATATCAACCAACAAACCATTCGACACCAAACGAA10.8849557522123894No Hit
TCCGAGATTCTTCGAGTCTCTCTGTAAATAATACAGCTGCCTTGGACGAAT10.8849557522123894No Hit
ACACACACACACACAAAAGTACAAAATTAGTAATTCATCTCAGAGGGACAA10.8849557522123894No Hit
CACCTCCTATGATAACAATGCCTTCTTTTGGAGTACTTCTTGAAGAACCTG10.8849557522123894No Hit
TGGGCTGAAAGCTCTCTTGTTCTGCATTTGTTGCCAGGTTTGAAATGGAGA10.8849557522123894No Hit
ACAACAAACCAAACACAACCAACAACAACCAACACCAAAAAACAAAAAAAC10.8849557522123894No Hit
ACACACAAACACTACAAAACAAACAAAACCAATTAATTTTCGAAAATAGCA10.8849557522123894No Hit
TGTGCTGTTATTAAGTGGAAATGTCTCGTGAACATGTGGAAAGTGATGACT10.8849557522123894No Hit
CCAGTTTTCAAGATAGAGAGAAAAAACACACGACAACGAACCTACAGCAGC10.8849557522123894No Hit
GCATAGTGCGTAGTGCTTTAGAAGCGATCGATCTTCAGCACAGGGAATGAG10.8849557522123894No Hit
AAAAACCAACAAAAAAAAAAAAACAACCCAAAAAACAAAAAAAACAAAAAA10.8849557522123894No Hit
ACTCAAAATTCAAAATAGAAACTAAAAAAAGGGAATATAAATCAGAATTTA10.8849557522123894No Hit
CCTTTTGTAGAAGGATCTGAGGGTGTTTTGGATTTCTTCAGGATCGATCTG10.8849557522123894No Hit
AAGAACAATAGCAACAAAAAATAAAAAAAAAAATGAACAATCAACCACAAT10.8849557522123894No Hit
ATTTCTTCCATACTCCTGCTAATGTTCATATTTCGACCTCCACCCATGAAT10.8849557522123894No Hit
TGTTGAGTTTTAGGACACCTGGGTGTGGTCGTGGGCTCCTTTAATCCTATC10.8849557522123894No Hit
CTTGTCCTGATTGCCATAGGGTTTGATTTCAGGCTTGTCTAACCATGCTTT10.8849557522123894No Hit
TAGATAGGGGATGTGATTAGAGATGATGACAATTGTTATATTTTGGAGTTT10.8849557522123894No Hit
AGTTCCTGCAAAGGACATGATCGATCTCATTCTTTTTTCTGGCTGCATAGT10.8849557522123894No Hit
CAAGTTCTCCTTCCCTGCTAATTTCTCCACAACTTTGATGTTTTCTTTATC10.8849557522123894No Hit
CAACAAACACAAAAAATCCAACACAAACACAAAGATCGAACAGAAAACCCC10.8849557522123894No Hit
CAAATGCTAGGATTAAAAGTACCTGGCCACCACCACCTGGCTAGGATTACT10.8849557522123894No Hit
CTCTACTTAAAAAAAAAAAAATTAGCTAGGCACACTGGTGTGTGCCTGTAG10.8849557522123894No Hit
GAGGCAGGAGAATCAGTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCGAG10.8849557522123894No Hit
CAACCAAAGTGTCCTGTCATGCAGGAATGTATGGTGATGACTAATAGATCG10.8849557522123894No Hit
TATAACATTCAATCAAAGAGTTATAGAAGATCGATCAAAACATAATGATAA10.8849557522123894No Hit
CTGTGGTACAGCAGGTGATCGATCAGACCAGGATGCAGCTATCTGAAGGAA10.8849557522123894No Hit
AAAAAAAAGAATGAATACGATCTATCTCAGGGCACACAGAACGAGTCTATG10.8849557522123894No Hit
GGAGTGCAGTGCCATGATCGATCTTTCCTGCTTTCTCTAATGGGCATTTAG10.8849557522123894No Hit
CATTTGTCAAAACTCACTATACATTTAAAATGACATTATCTTATTATATAT10.8849557522123894No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT10.8849557522123894Illumina Single End PCR Primer 1 (100% over 31bp)
GGGCGCGTAGCCCGGAGTCCCAGTGTGGCCCGGAGGAACGGAGCCCGTGCC10.8849557522123894No Hit
TAGATAAGATATCTGGTCACAAAAAAATATTCAAATCTTCATGATCGATCC10.8849557522123894No Hit
CTTGGCACTGGCACCGCTGATGCCAACCTACAGGGAAGGAAAGTGACACCC10.8849557522123894No Hit
GGTACCAAAACAGAGATATAGACCAGTGGAACAGAACAGAGGCCTCAGAAA10.8849557522123894No Hit
AACTTGGCTTTTCTTAACAAGCCATTCTACCGCCTTTACTACTTTTGTCCT10.8849557522123894No Hit
TCCTGACCTCGTGATCCATCACACTTTTAAGTCTTGAAACACTGATGTATT10.8849557522123894No Hit
TGATGAACACCCAATTGAGTGTTGACAAGAGAGGTGCTGAGAGAGAAGGGC10.8849557522123894No Hit
AGTGGACTCTACTTGTAAGAAGCTGCAGTTCTAACCAGGAGGGAGTGATCG10.8849557522123894No Hit
TAGGAAGAATCAATCTTGTGAAAATGGCCACACTGCCCAAGGTAATTTATA10.8849557522123894No Hit
GGAAAAGCAAGAGTCAGATCGATCAGGGCTGCCTCTGAATAGATTGTCCTT10.8849557522123894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8849557522123894No Hit
CTATGATCCAGCCACTCCACTCTAGCCTGGGTGACAGAGAGAGAAAGAAGA10.8849557522123894No Hit
CAAAATGGAATTGTTTGGGTCAGATCGATCTCAGCTCACTGCAACCTCTGC10.8849557522123894No Hit
AGTGTGATAAGGCACCCATAGGTAAATAACACTCCTGCTTTCTCTCCTAGG10.8849557522123894No Hit
AAAAAACACAAACAACAAACAAACAAAACAAAACAAAACCAACACAAAAAA10.8849557522123894No Hit
AAACAATCAACAGAATCAGCATCAGCACCTAAGTTCTCTAAAGCTGCAAGC10.8849557522123894No Hit
CGGCTAATATTTTTGTATTTTTAGTAGAGACAGGTTTTCATCATGTTGGCC10.8849557522123894No Hit
CTTACTGTCTTTCAGATCGATCACCTGAGGTCAGGAGTTCAAGACCAGCTT10.8849557522123894No Hit
TTATCCTCTGTATTTTCCATATGCAGTTATAAGTAGGTTTCATGGTTATGT10.8849557522123894No Hit
AACCACAACAAAGCAAAAGCAAGCGGGCAGGACACCAGAAAGACCAACGCA10.8849557522123894No Hit
TGAGAGGGCTGATTCAGCATCAGTAGCCCTGGCTAGATGATCACGCTATTG10.8849557522123894No Hit
TTCCATAAAATTGTATGGTTTTAATAGATCGATCTCTTTACTTTTCAACCA10.8849557522123894No Hit
ACTTGCAAAGGATATGATCGATCAGAGTGATCTGTTATGTTAGAAACATTT10.8849557522123894No Hit
CCCACACCAACCAGCCCACACCCCCCCCCCAGCTAAAACTCAACGACACAA10.8849557522123894No Hit
AATTATACCCTAATGCCAAAATATATTTTGACATAGGAGATATAAGCAAAA10.8849557522123894No Hit
CTTAGTTTTCCTGTTAGGTTGTGAGGTCTTTGAAGGCAGTAACAGTGGAAT10.8849557522123894No Hit
AAAATGATAAAACCATTATTTTTAATAGGGGAAAGGGAGGTGGGAAAAGGT10.8849557522123894No Hit
TTCTTAATGTTTCTGTCATGCTGGAAGTGTTTTTCATGCTTTGTCAAAGAT10.8849557522123894No Hit
GAAAGCAAATTGAGCAGTCCATTAGGAGAAAGCCAATAAGCAGCATCCCTT10.8849557522123894No Hit
CCCGCGCCCATTGGAACTCACAGGCTCGGTCAGAAATCAGCAATCAAAACC10.8849557522123894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)