FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n02_MCF7_DMSO_12_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n02_MCF7_DMSO_12_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTTAGGGAAATGCAAATTAAAACCATAGTGAGATACCACTTCACACCT21.8691588785046727No Hit
TAAAGAAGAACAAGCTGGCCAGGCACAGTGGCTCACACCTGTAATCCCAGC21.8691588785046727No Hit
TACAATTATGCAACAAATAAAATGGAATCTTCAATAAATAGCATACTATTG21.8691588785046727No Hit
AGGGTCTGGGAGAAGCAGGTCAACAAGGGAACAGCAGGGGCAAAGGTCCTG21.8691588785046727No Hit
TGTCTTTCGCAGCTGGGAGGCAGGTAGCCTGGGGCAAGTTCTCAAGACCAG21.8691588785046727No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGAACTGCTTGAACCCAGGAGGCAGA10.9345794392523363No Hit
TGATACTTACCAGGATCACTCGTCTAACCTAACTTGATAAACATCTGCAGC10.9345794392523363No Hit
AGGATGCTTTTGTGCCTCTGTAACAGAGGCTTCTAGACTTTCTCCTGGTTA10.9345794392523363No Hit
AGCCAATTTTGTCAGATATAAAAATAAAACGTTATTACCTGATTCCACAGA10.9345794392523363No Hit
AAGCCAAATGGCATCCCTCTCTTCCAGTCTTCTCCATCAACCTCTTCAGTC10.9345794392523363No Hit
AGAGGAGTGCCTGAGCTCAAGAGTTTGAGACTAGCCTGGGCAACATAAGAT10.9345794392523363No Hit
AGAGGAAGGGGCGTTCTCCTCCGGGATATGGGAGATGGTGTGCCTCAGGTG10.9345794392523363No Hit
GATCCAAGTAGGAAATTTACGCATTAGAAGGTATATGTTTAAGATCGATGT10.9345794392523363No Hit
TGAATCCTATTAATTTTCATCTCTGAGTCAACAAATGATCGATCTGTAAAA10.9345794392523363No Hit
AAGCTTTTCATATCTCCCTTCTCTGTTTTTCTGTTTTTTGTCTCCCTCACA10.9345794392523363No Hit
AACCTTTCCTGCCACCAGAGGTATGCTAGAAGAAAAAAAAAAAAAAATGAA10.9345794392523363No Hit
AGCCATTTCCTGGAAGAAACATTTTGATTGACTAAGTAAGGGCAGAGTAGC10.9345794392523363No Hit
TCAGCATTTAACTCCTTTTCTAAATAAATCAAAACAACTGAGCCTATTTTG10.9345794392523363No Hit
AGGCCCGAGGCTACCTTTTACTTCTGTGTTTTGTCTTAGTGACAAGAATAG10.9345794392523363No Hit
TGTGAATTTGATCGATCTGGCGATGTGGTTGGACTCGGAATGAGAAAGGTT10.9345794392523363No Hit
AGCAGTGCCCAGCATGGAATGAGGCCTGCTTCATCCCACCCTTGCCCTGGT10.9345794392523363No Hit
GTTCTGGTGTATATACCCTGAAAGTGCATTCCAGGACCTCAGAGGCATTTC10.9345794392523363No Hit
AATCGACAAAACTTAAATTAATAAAAAACTTTAAAAAACAAAATCCAGTTA10.9345794392523363No Hit
TAGACCAAGAACTCAAAGGTGCTCATGAAGCAATCTGAACGCAGAATCCTA10.9345794392523363No Hit
TCAGAAACGACTGGCTCTTCTTATATCCACCGTGGGTCTGCTTTGAGACCC10.9345794392523363No Hit
CAGCATCATAGTTTAAAGTTTTGCCTCTCCATCTAGAACTTATAACTCAAT10.9345794392523363No Hit
AATTTTACGGGTTGAAAATTATTCTTAAATAAAATTAAATTTCCTGAATAG10.9345794392523363No Hit
TGGCTTTTCCAGGCACACAATGCAAGCTGTCGGTGGATCTACGATTCTGGG10.9345794392523363No Hit
ATCAGTCTGGGGGGTTGGGGGGGGGGGAGGCGTTCCAGGACTGCCTTAGCC10.9345794392523363No Hit
GTTTCAGCATGAAAGGAAGACTTATTAATAATTTCAGTTAGCCAAACATTA10.9345794392523363No Hit
GTTTTATTCCCAAAACTACTCTATCCAAATTGCAGGTAGTGGACCAGGAAT10.9345794392523363No Hit
AAGAAGGAGTTTCACTATGTTGGACGGCTGATCGATCAATACTGGGGACGT10.9345794392523363No Hit
TGTTTAGAAACACATAATTAAGTGTTTACATTCCGTACTAATCAGGGACAT10.9345794392523363No Hit
TATTGAGTATTTTTGTTTTCTTATATTCTCCATAGTAATGCTTTTTAAAAC10.9345794392523363No Hit
TATTGTTGCCCTGTGGTCAAATCATCTAAACCAACCACCACCTGGAATAGT10.9345794392523363No Hit
TCATCTGCCTGGGTCCTAGCTGTTCCCAAAACGTGTTGTTTCTCTTCTGCT10.9345794392523363No Hit
TATCAGATAACTGACCAATTTTTGAAACTTCAGAAGGTATTTTCACTTTTC10.9345794392523363No Hit
TAATAAAAGTGCCACTAGCACGTCTGTAGTTCTCTATAAAGTAAGAATTTT10.9345794392523363No Hit
TCCATCCATGCCACTCACTGTTTGACATTGATCGATCCAGAAATCTCAAAA10.9345794392523363No Hit
ATGTAAGGTGGTCAGAGAACAAACCTATGAGAACCAGTCCTCCCTTCCATC10.9345794392523363No Hit
AGGAATAAGGCATAGCAAAAATAAAAAGTTGCATATTTTGTTGTTTTTATA10.9345794392523363No Hit
TGGGGAAGTAGACACTGGTGGCTTAGAGGTTAGGTATCTGCCCAGCCCTAG10.9345794392523363No Hit
TGAAATCAACCAAGTGGAAACAAGAAGAACTATTCAAAGAATCAACCAATC10.9345794392523363No Hit
ATAATCATGTGGTTTTTGTCTTTGGTTCTGTTTATATGCTGCATTACATTT10.9345794392523363No Hit
AATCAGTGAAAAACATAACATGAAGGACCATAATCATCAAAACATCACCAT10.9345794392523363No Hit
ATTATCAATCTAATCTATACAGCAAACAAAACACAGACCTCATCTTAAAAA10.9345794392523363No Hit
TTTGAAAAAAACTTGTTATATTGTATTAGGTCGCGACTTAATAACCTCAAT10.9345794392523363No Hit
ATTGGTAAAAACTAATCAGTAAATTGAGATGATATTTGACAATTGTGTTTC10.9345794392523363No Hit
TTTTTATTACGTATTTTCCTCAATTACATTTCCAATGCTATCCCAAAAGTC10.9345794392523363No Hit
GTTTAAGCCATCCTCTCACCTCAGCCTCCTGAGTAGCTGGGACCATAGGCA10.9345794392523363No Hit
TGCAATGCAGTGGCACAATCTCAGCTCACTGCAACGTCTGCTTCCCACGTG10.9345794392523363No Hit
TAAGTCCCGACCGTCGACAAGGCGACTTTCCCGGGAGGAATCCCGGCCCGG10.9345794392523363No Hit
TATTTTTAAAACCTTAGAAGAATCAAAAAAAAAAAACTTAGAAGAATCACA10.9345794392523363No Hit
CTTCATAAACTTATCAGCCTGTTGTATGTCTTTTTTGAGAAATGGCTGATC10.9345794392523363No Hit
TACCACAAAGCCAAAAAAATCAGACGTGCGATGTGCCAGACGCATGTAATC10.9345794392523363No Hit
CGTCTCTGCTCAGTTGAGTGAATCATCTCTACTCAGTTCCGTTCAGAACGT10.9345794392523363No Hit
GCTCACCACCACCTCTGACTCAGGAGATGGCAAGATCATCCAGAAAACCGA10.9345794392523363No Hit
TGCCACTGCATTCCAGCCTGAGTAACAGAGCAAGACCCTGTCTCAAAAAAT10.9345794392523363No Hit
GAGAATGAAGACTGAAGTGTGGACACTATGCCCCTCCTTAGATGTGGGAAC10.9345794392523363No Hit
TAGAGACGGGGTGTCACCGTGTTGCTCATCTGGTCTCAAACTCCTGAACTA10.9345794392523363No Hit
GCACATTTGCATGCCAAAGAGGCACTATCGATCTCACTTCCATCCACCACC10.9345794392523363No Hit
TGACATAAAATCACATAGCGTGCAAAGAACCCCTGATACACACTGTATTTA10.9345794392523363No Hit
TTCCTTCATTTCTTTTCAAGTAGAGGCTAGGCTAACCTGCCTTCTCTGTAG10.9345794392523363No Hit
AACAGTGGCCTGCCCTTGAGCCCATGAAGGCCTTGTCTTTGTTGCAGTGCT10.9345794392523363No Hit
AGGCTGTGCCCACAGGGGGATTGAGACATATCTCTGCACTGATCGATCTCG10.9345794392523363No Hit
ACCCAAGTTCAACTGAAATACGGTAAACAAATCAGTACTAAACATACCATA10.9345794392523363No Hit
GCATTTGTAGCTGGGTAACCAGGGTATTCTTCATACAGCTGCAAGTGTCTG10.9345794392523363No Hit
CCGCTGACACCCGGCAGCGCATCCACGAGTACTACGAGCATCGCTACCAGG10.9345794392523363No Hit
AAGCATCTTGATTAAGAAATAAGGAGTTAGTTAAACTCTAGTCCTGGGCCG10.9345794392523363No Hit
TGTAACACTAAAATTTTTCGATGATCGATCCGAAGATGCTTTTGTAGGCGA10.9345794392523363No Hit
AAACAACAAACAACCAGATGCTGGCAAGAATAAAAGAAAAGGGAACTCTTA10.9345794392523363No Hit
TTCCATTTTAAGAGCCTTAATTTGGCTGGGTGTGGTGGCTCACAACTATAA10.9345794392523363No Hit
ACCAAAATTGAATTCTATTAATAAAAACTCCAAATAACTACAAAAATACAA10.9345794392523363No Hit
TCATTGGTGTCTTGAACTCTTCTAAGCATTGTCTAAGTGTCAACTTTGTTT10.9345794392523363No Hit
CACAGAAGGGGTCAGCATACAACCAGCATGTTTGGGCTGCCAGGACCACAC10.9345794392523363No Hit
AAAATGTGAACAAGCTTGCTAGAGGGTCTAATTAAACCATCAACTTCAAGC10.9345794392523363No Hit
TGCCAGAATAATTTGGGGAAAATACACATTAGCTAGGAAAGTCCACACATT10.9345794392523363No Hit
TTGGCATTTCTACCTACTTGAAAACTTAGAAATCTAACACTGTGTCATATG10.9345794392523363No Hit
AGCCTCCCAGAGTGCTGGCGTGAGCCACTGCACCCGATCGATCATTGTAGA10.9345794392523363No Hit
CATAACTAATTTAAGATGGAACTTTCTCTTAATTTGTATCAAACATCAGGG10.9345794392523363No Hit
TGACAGAGCAAAGAGATCGATCGTGCCACCGCACTCCAGCCAGGGTGACAG10.9345794392523363No Hit
AAGTTTCTCAGAATGCTTCCGTGTGGTTTTTAGATGAATTTATTTCCTATA10.9345794392523363No Hit
GGCAACAAGAGTAAAACTCCGTCTCAAAAAAACAAAAAGACTATTTACTTG10.9345794392523363No Hit
CGCCTGGCCAAAGTTTTACCATTGGCCCCTACAGGGATCAAACCTGCGACC10.9345794392523363No Hit
TCGCTGTGTCTCTGAAAGGCCCTAGTAGGATGATCCAGTCCGGAGATCGAT10.9345794392523363No Hit
TTACCATGTCATAAGGAATAACTCAGACTTAAGGGTCAAAAGACCGAATTG10.9345794392523363No Hit
TTAGACTATTTTATAGCCCTATTTATTCATTTATTACTAGATGATTTCTGT10.9345794392523363No Hit
TAGTCCCAGCTACTTGGGAGGCTGAGGTGGGAAGATCGATCCGACACTGAC10.9345794392523363No Hit
CGCCCGGCCTACACCAAAGTATTTTGAATAACTGATCGATCTTCAGAGCGT10.9345794392523363No Hit
AACCACATAGTAGAATTGCCTGTAGTTATCCACACAGACTGTTGCTTACGT10.9345794392523363No Hit
TGCAAGGGACATTCCAGAAAAGCAGTTTGTTTAATTTTTTTGTTTTTAAAG10.9345794392523363No Hit
AACCTTTCCTGCCACCAGAGGTATGCTAGAAGAAAAAAAAAAAAAAAAGAA10.9345794392523363No Hit
AAGTGAGGAACAAAGAAAACAGTCAGCGCAGGGCACGGTGGCACATCCCTG10.9345794392523363No Hit
TGTGTCTTATCTCTGGACAAAGCCTCAAACAGAAGTTTGATATCAATTTTT10.9345794392523363No Hit
TGGTATGACATTTCCTGGCACAGACCAACGCAGTATGGAACCACTTGGCAT10.9345794392523363No Hit
GGGGAAGGTTCACACCAAACCCTGCCCCTTCCCACCCTGATCCCTCAGACT10.9345794392523363No Hit
TGAAACAATGCATTGTTTCTCCTTTATTTAGTGGTGGGGATTATGAATGAC10.9345794392523363No Hit
GGAAATAACACAAAACAACACACCTCTAGTTAAGTGTGGGGAAAACCTTGT10.9345794392523363No Hit
TGATTTGTAAGATCGTCAGGGATAATCTTAAGTTGTTCATTGTTCTTACAA10.9345794392523363No Hit
TAGCTAGACTAAGAAAAACAGAGAGATAACCCAAATAAATAAAATCAGAAA10.9345794392523363No Hit
GATCCCCGACCGACCCATGCATGCTGGTTGGCCCTGGCTTTCCCCGGTCCT10.9345794392523363No Hit
AGGCCGAGGCAGGGGGATCGAGCATCTTCGTCATTCTGATGATATGCGATC10.9345794392523363No Hit

[OK]Adapter Content

Adapter graph