FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n01_MCF7_DMSO_22_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n01_MCF7_DMSO_22_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCGGACATGGTGGCGCACGCCTTTAATCCCAGCACTCAGGAGGCAGAG33.7037037037037033No Hit
CATAAAAATAGGTACATAGACCAATGGAAAGAATAGAGAACCCAGAAATAA22.4691358024691357No Hit
CAATTATAATTTTCAACTCCAGAATTTTTGTTTTACTCATTTTTGTATTTA11.2345679012345678No Hit
AGTTATTGTTTCACAAATGTTTACTATAGCATCTCCTTGTATTTATTCTCA11.2345679012345678No Hit
GATCACTTCATTAGTTGCAGAAAGACTTTAACAAAACCTAACATCCTTTTG11.2345679012345678No Hit
GCAGTGTCAGTACGGAAGCCACCAGGTGGATGATCGATCTCCGTTCACTGC11.2345679012345678No Hit
TACATAATACCACATAAAAACATGCAAATACTACTCACAAAATACACTCAA11.2345679012345678No Hit
CTTTGGTTGTAACAAGAACTAACAATTACAAATTAAAAATAATAAAAATTA11.2345679012345678No Hit
TTTTGTTCCAGTGTTGTGGAAGGTGGCAAAGAAGGAAGCTGGGATCGATCA11.2345679012345678No Hit
TATTCCACGATTTTTTGTGGTACTATTATATCACAGTGAAAAATAATTTCA11.2345679012345678No Hit
GTACCTATTATAACCAAAAATTTATTAATAATGAATACAAAAGAAAAAACT11.2345679012345678No Hit
TCAATAGATACTGAAGAGATAAACATGATAAAGCACAAAGATCGATCCTAA11.2345679012345678No Hit
TGCCTTTAGAAACAATAATAATAGGGTGAAAAAGTGCCAAGCTAAGAAATA11.2345679012345678No Hit
GGCTGTTATCAAACTACTCTCCAAAAGAAAAACACTATCCACAACCAATCA11.2345679012345678No Hit
ACCTGAGTGTCCGGGGAGCATCTGCCTTCACTCCAACTGAACACTTGACAG11.2345679012345678No Hit
AACAATTTTGAGGAAAAGAAAGGCGCTTTGGTATAACTGTTTGTGTATACT11.2345679012345678No Hit
AGATAAATTTTTAATTTTAATAATTATTAAAAACAGGTATACACAACCTTC11.2345679012345678No Hit
GCATGGTGGCAGGCGCCTGTAATCGCAGCTACTCAGGATGCTGAGGCAGGC11.2345679012345678No Hit
GTCACCTGTAGTAGCTGCTAGGGATACTCCACCAGATCGATCCAAGTAAAA11.2345679012345678No Hit
CCAAGCTTGGGGTAAGAGAGCTGTGACTGCATACTGGTTGTGCCACTTGCC11.2345679012345678No Hit
AGAGTAACACTTCTCCTACAACTCAATAACCTAATATTGAGATGTTCGTGT11.2345679012345678No Hit
GGCACGGACCTTGACCTTGATCGATCAGTGTCCCTCCAAAGCCTCAGGCCA11.2345679012345678No Hit
AAGATTGGTGGGTGTGTGGATGGGTGGGTGGATGGATGGATGGATGGATGG11.2345679012345678No Hit
ATTCCACAAAAAGAGTGTTTCAAGTCTGCTCTGTGTAAAGGATCGATCAGG11.2345679012345678No Hit
TCCCAAGTCCTGGAGCTAACCTGGAGAGAAGCATGGAGGCACTGAGAGGAA11.2345679012345678No Hit
ATGTCATCTTACAGGAGTGTTCTCCAAGACATGACGTGGATCGATCCCACC11.2345679012345678No Hit
GACTACAGGCGCCCGCCACTACGCCCGGCTAATTTTTTGTATTTTTAGTAG11.2345679012345678No Hit
CCTCACAATCATGGTGGAAGGTGAATGAGGAGCAAAGCCAAGTCTTATATG11.2345679012345678No Hit
CACCTGGGTGCAGGTAGGCTGAGTCCGAAAAAGGAGTCAGCAAAGGGGGGT11.2345679012345678No Hit
GCCTCCCTGGTTCAAGCAGTTACCCTGCCCAGCCTTCCGAGTAGCTGGGAC11.2345679012345678No Hit
AGAGACCGCGGCGACCTATTATGATAACCTCGAGAACCAATGAAAAACGGG11.2345679012345678No Hit
TGAACCAACTTATATAAGTCAACAGCATATCAACATATTTGTAATTTATTC11.2345679012345678No Hit
CAGAAATGCAAATCAAAACAACCCTCAGAATCCAAAACACACCACTCAGAA11.2345679012345678No Hit
AAAGATGAGAATGGAAGGGTACTGTTTAGGAAGACAAAGAGGGCCCATGGA11.2345679012345678No Hit
CAGGACGTGGGTGCCCAAGTAGCCCAACAAACAGTCCCCGGAGAGATAGTA11.2345679012345678No Hit
TGTCACCCAGGCTGAAGCACAGTGGTGCGATCGATCACCAGTGTTAAACCC11.2345679012345678No Hit
TGTTCTCTCAGGCGCTGTTAGCATGAGTGTGTGAAGGCCTTGGCTTCTGCC11.2345679012345678No Hit
GCAGAAACCTTACAGTGGAGACAATTCAATCATTCTCAGTAACACTAATTA11.2345679012345678No Hit
TGAACATTAAATTACTAAAAAATAAAAAAAATAATTAATACTTTATACCAT11.2345679012345678No Hit
GTTTAAGAAACCCCATACAATAAATGGATATAAATACATACAACAAAAAAT11.2345679012345678No Hit
GCAGTGGCCCGGGGGCTGCCCGCGCCTGGATACTCGCTGCTCCTGGGTATC11.2345679012345678No Hit
TAAGTTCAGGAGTACATGTGCAGGATGTGCAGGTTTGTTACATAGGTAAAT11.2345679012345678No Hit
ACCACCTCAAAAATCATACAATCAAACAAATTGTTTGAAACATACAATAAT11.2345679012345678No Hit
ATTTAATGAAGACTTCTTTGTGAATCAAGTGCTAGATGAGACTGCAAATTT11.2345679012345678No Hit
CTGATGACTTTGCTAGGCACTGGTCCCAGCACACCCGGCCAGCAGGACATT11.2345679012345678No Hit
AGCTTCTGACCATCCTGGCTAACATGGTGAAACCCTGTCACTACTAAAAAC11.2345679012345678No Hit
TGATACCAGTATCATCCTCAGTCAACAAACGATAGATAACTCAGAGAATGA11.2345679012345678No Hit
AAATAATGAATAACAAAGTAAATAAATCCATCAATACCAAACATTTTTTAA11.2345679012345678No Hit
TAGAAGGGCATCCTGCAGTAAAGAGAATGATGGTGGACTGTGACTGGGAGA11.2345679012345678No Hit
TCCCAGCCTGGATCGATCAACCGAATCATACAGTTACAAGCTGTCTTAGAA11.2345679012345678No Hit
AAAAAAATCTCACTCACAAGTCCAACACCATTCACAAAACAACAAATATCC11.2345679012345678No Hit
TAAATGAAAAGATATCCCATGTTCATGGATTGGAAGAATTAATATTGTTAA11.2345679012345678No Hit
TACACACCACTGGTCCAAACGTCTCCACTGTCTTGGGCAACATCTGGAGTC11.2345679012345678No Hit
GGCTGGTGTGTGCAATGGCATGATCGATCCATGAACATGGGATGCCTCTCC11.2345679012345678No Hit
TATCAGTTGTCTTTTAATTACAAACATTACCCTCATCAAGGGAATTATTAT11.2345679012345678No Hit
GTGTGTTCCTTGTTCATTTGGAGTCTCTCCACTTGCCTGAGTGCTGAGGGG11.2345679012345678No Hit
GGAAGTGAAAACATTTCTCACAAACATTTGCATGAAAATGTTCACAGCAGC11.2345679012345678No Hit
CCACATCCCTAGCCGTCTGCTTCCCTGATCTTAGCCTTATTTGGTTCATTT11.2345679012345678No Hit
TAAGTCCCTTATGGCTATATCTGTCATAAACAGTGCTTCTTTTGATGGATC11.2345679012345678No Hit
AATGAATCTTAAATGCATTAAGGTAAGTGATGGAAGTCAGACTCAAAAGGC11.2345679012345678No Hit
ACATAGACCAATTCAATGTACAGAAACAACAAAGAAAAATACTCCATCCAA11.2345679012345678No Hit
CAATCAAAAAAAAAAAAAAAAAAATAAAATGGGCAAGGATGTGTGATCATT11.2345679012345678No Hit
TGAACATCCTTGATCGATCCCTGTGTGGAGGAGGTGTGTTGTGTGGTGCTT11.2345679012345678No Hit
CGGAACAATACAAGCTCTCCCACCCTATTTCAACCGAAAAACAACACTCAT11.2345679012345678No Hit
AGAAAGGGGAAAATACTAGAATAAAATTTTAAGAAATATTGAGAAACAGTG11.2345679012345678No Hit
ATACGAAACAAATTTTAAACACTTTAACTAAAACTACTACACATTATATAT11.2345679012345678No Hit
ATACCCTGATGAAGAACTTAGTATTTTTGGAAATAATAGTTTGTTATTTGA11.2345679012345678No Hit
GTGAAAATTTATATAATATTTTTAAGTATTAGAGACTCAACAACTATATCA11.2345679012345678No Hit
AGTCATTCAAAAGAAAGTATGGTGAGTCCCAAGATCGATCTTATCTTCAGA11.2345679012345678No Hit
GAGCCTTTAAGAAATGAGTATAACAAACATGTACAAAAGGATCGATCCGGG11.2345679012345678No Hit
ACCCAAATCATCAATAAAGACAAAATAAAAACAATAAATTGTCAATTCAAA11.2345679012345678No Hit
AAAAGCACGGAACTGGTCCATTAGGACAACATTGCTGCATAGTAAGGAACC11.2345679012345678No Hit
TTATAACAAAATTATTAAAAAATAATAGAACTTTGATCAAATAACCATTTA11.2345679012345678No Hit
GGTCATTAGAAGGCAGTTAACAAACAAAACATTAATAAGAATTTACTAAGA11.2345679012345678No Hit
TAATCTCTAGATTATTACTGTAGTATGGATGATACTGCCAGTTCCCAAATA11.2345679012345678No Hit
ATGTTTCTGGAAGGTATATAGCTAGAAAAGAATTGCCAGGTCACAGTGTAC11.2345679012345678No Hit
CTCAAAAAAATTAATAAAAAAGAGATACAATTAAACAATTTAACCACGAAT11.2345679012345678No Hit
CAGGCACCAGGGGGCAGCAGTGGACTCAGAGGTAGAGAGTCTAGATCGATC11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)