FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n01_MCF7_DMSO_21_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n01_MCF7_DMSO_21_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACCCGGGACCCAGGACAAGGGGAAAAGAAACATCTTCAGACACGAGTG32.6548672566371683No Hit
GAAGTAAATACACACAAGAGGAGTAGATGGCGGGAAATAATCAAAGTCAGG21.7699115044247788No Hit
CCAGAACCACCTCAGTTTACATCTCAACTTTACCACTCTCAGTGAGAAACG21.7699115044247788No Hit
TGACCGAAGAGGAGAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTT21.7699115044247788No Hit
TGAAAAGAACTACAGATGAATTATAGGTAGATAATATGTGACAACTGCACG21.7699115044247788No Hit
ATGTTTTTTATGTGCTTGTAAGGTAGAAGGAGTCACAGATATAGGGATAAG21.7699115044247788No Hit
GAGATGGGGTCTGACTTCTTATTTTAAACGTTTATAGCCAATTTTCTTAGA21.7699115044247788No Hit
TCCCTCATTCTCCTAGCCTCAGGCTCCTTTCATAGAGTCAGCTTCACCTAT10.8849557522123894No Hit
GTCCACTTTGGTATGTTAAATATGCACCTCAGCTGTTGGTCCAGGGTCCAA10.8849557522123894No Hit
TACACACCTTTAGTCCTAGCTACTTGAGAGGCTGAGGAGGGAGGATCGATC10.8849557522123894No Hit
GCAAACACACAAAAACCAAAACCACACAAAAAAAAAAAAAAAACCCCCAAA10.8849557522123894No Hit
CTCAGCTACTTGGGAGGCTGAGGCAGAAGAATCACTTGAACCCAGGAGGCA10.8849557522123894No Hit
CCTGAAGTGATGACAAAGGTGATGTAACCCTTGCCTAGGCTTTGCCTACAG10.8849557522123894No Hit
AAAATAAATATCAATTATAAAACATATTACAAATAAAGTAAATAAATATAT10.8849557522123894No Hit
AGCCTGGGTGACAGCGAGACTCCATCTCAAAAGAAAGAAAGAAAGAAATAG10.8849557522123894No Hit
AGCACTTTGGAAGGCCGAGGCGGGTGAATCACTTCAGGTCAGGAGTTTGAG10.8849557522123894No Hit
TCTATGGACATCAACCAGCCCCAGCATATCAAGCCACTGCAAGACTAGGCA10.8849557522123894No Hit
AAAACAACATGATATAACCATCCAAAACACCAATCAAATAACAAAGAATAA10.8849557522123894No Hit
TAGCACAACCTGCAATGGCTCCCACACATCACTCACTGTCTTTTTTATCTT10.8849557522123894No Hit
TCTGTAGATATCTGTCAGATCATCCATTGTTATGAGAACTGCCTGAATGTA10.8849557522123894No Hit
GTTCTTATTACATGTTGGAGCATCTTCTGAGTATATGCCCAGGAGAGGAAT10.8849557522123894No Hit
TAGGAAGAATCAATCTTGATGGGGATGGCATTGAATCTATAAATTACCTTG10.8849557522123894No Hit
AAAAACCAAACAAACCAGCAACAACAACAGAGACCAAACAAACCAACAAAA10.8849557522123894No Hit
TGCAACAACGATAAAAAAACTGAGATTCACAAAAAACAAAAAAAAAATAAA10.8849557522123894No Hit
AGGGAGAGATAAGGATCGATCCAAGGGGTTTAAAATTCCTTGAAAGCATCC10.8849557522123894No Hit
TCAGAAAATCACACATACAAACACACAAAAAAGAAATTTGATCAAAACAAT10.8849557522123894No Hit
CAAAACAAACAAACACACAAAAAAACACAAAAAAGACACACCAAACACCCA10.8849557522123894No Hit
AACAACACAAACACAACAAAACCAAAACACCAACAAAACCAACAACAAAAC10.8849557522123894No Hit
GCTAGAAGAGGACACACAGAATAAGGGTACCAAATGTGTTAGCCACCCTCA10.8849557522123894No Hit
CCAGGGAAGAATAATAGGATTTTTAAAACTATGGAATTGAGGAAAATTAGG10.8849557522123894No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTATGTCGTGTTGGGGGCAGGGAGCAA10.8849557522123894No Hit
TAGACATCATTTCATTGTAAAAGCCAAATAAAATTTTAAATGTGTGTTGAA10.8849557522123894No Hit
TTTTCTTTTTTTCTTTTTTGAGATGGAAATTCACTCTGTTGCCTAGGCTGG10.8849557522123894No Hit
AAAATCATACTGTGAGTTGCAGTTTACAGTTTGAATAGATCGATCATAATG10.8849557522123894No Hit
AAAACAAACACAAAACAACACACAACACCACAAAAGCAAAAAAAACACCCA10.8849557522123894No Hit
AGGCTGAGGTGGGAGGATCGATCCTCCCAAGAACCCTGTGTGGCAAGTACT10.8849557522123894No Hit
TAGACCTATATGCCGGCAACTACCTAGCCTTCCCACTAAATACCAGAATTA10.8849557522123894No Hit
CTACGTGGCCTCAGCCTCCTTCTCCCACCTTTAAAGCATTTTTTGAGTTCC10.8849557522123894No Hit
ACTTAAATGAGTGTGTTGTTTCCTGATTAAAATTGATTCAGAATAATTATA10.8849557522123894No Hit
CCTGGCCTCTGGCTAATTTTTTATTATTAGTAGAGACGGGGTCTCACTATG10.8849557522123894No Hit
TTTTTTTTTGAGACAGGGTTTTTCTGTGTAGCCCTGGCTGTCCTGGAACTC10.8849557522123894No Hit
TCCCAGATTTACAGCCAACTTTTAAATCGCAAGAACAAGGATAGGCTGATC10.8849557522123894No Hit
TNCTATAAAAAAGTTCTAGTTTCCACTTGTTATGATAGTTCAATGATCGAT10.8849557522123894No Hit
ACATCCAAGTTCTACTTTCCACCAGGGATCGATCACAAGAGGTGCTGATTG10.8849557522123894No Hit
ACCCAAACACCAACAAAAAAAACACCACCCCACAACACCCACAACACACCC10.8849557522123894No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACAAAAAAAAACAAAGGCA10.8849557522123894No Hit
CCCACCCACACCAAACACACACCACACAACCAACAACCCACAAAACACCAC10.8849557522123894No Hit
TCCATAGTAACATTTGCAGTAGGTTGGATTTGGTCATTACATCTTGCAGTA10.8849557522123894No Hit
AAACAGAATTGGTTAAAAATCTTTAGATCGATCTGGCAAAGTAAATTGAAA10.8849557522123894No Hit
TGTGAGGTTGGTGTCAGGGATAGCAGACCTGGTACTGGTGCTGGTGGGGGC10.8849557522123894No Hit
CCATTGTAGAAAGCACTGTGGCGATTCTTCAAAGAGCTAAAAACAGAACTA10.8849557522123894No Hit
TATTGAATATAAGAAAAAATTTAGGAATTAGTAATGTGTCATCATACGATC10.8849557522123894No Hit
AGGAGAAGACGTAAAACAGTCATCGGGCCTCATTTTAAAGGCCTCAGATCG10.8849557522123894No Hit
ACAACAAAAAACACAAACCAACAAACAAACAAAAAAACAAAACAACAAAAA10.8849557522123894No Hit
TGCAATGCTGTAATCCTAGCCCTGGGAGAGGCTGAGGAAAAATGGCTGTGA10.8849557522123894No Hit
AAAACAAAAAACACCAACCAACAACAAAACCAACAACCCCACACCCAAAAC10.8849557522123894No Hit
GGAAACAAATTTTCCCCTAGATCGATCTGATGGAATCTTTGTTTCCTATTC10.8849557522123894No Hit
CTCTTCACCTTGTGATCCCCTTGCCTTGGCCTCCAAATTTGCTGGGATTAC10.8849557522123894No Hit
GACGGTCTCACTTTCTCACCCAGGCTGGAGTGCAGTGGCGCGATCGATCAC10.8849557522123894No Hit
GCCAGAGGGGTCTGGGAAGTCCCAAAGGGCATGAGCAGCTGGGATTCATCC10.8849557522123894No Hit
TGCCAGAATCCTTTGAATTGTTAGTAAAGCAAGTGCCATGTTTCCAGTGGC10.8849557522123894No Hit
TGCCTGACACATAGACAGCTCTGTTCAAATGCTCCTTGGGCATCCTGCACT10.8849557522123894No Hit
ACCAGAAAAGAGTAATTTGAGTAGGAAGAAATGCCAAGTCACTAATTTGTT10.8849557522123894No Hit
TTCTTTCCAGGAAACAAGTTTTCCTTATGTCAGTGGGGTGGGCAGGAACAT10.8849557522123894No Hit
CCCAAGGGTTTAGCCAATAAGGTTCCCTTCCTGGACAATAAATTTTGATCA10.8849557522123894No Hit
AAGGCATTTCCTCTACTGCAAAGCAGACTACATTAACCCTACTAAGCAGAA10.8849557522123894No Hit
GCCTTCTCATCTAGAATATGACCAGGATAGATAAGGCAGAGGAGTCTATTC10.8849557522123894No Hit
GATTTCTAAACAAAGTGCCACAATTAAAAAAAAAAAAAAGGTCAGCCAGGC10.8849557522123894No Hit
AGTCTGAAGTCAGTTTCACTTGGTCAAAATGAAAGTCTCAGAAAGGCAGTG10.8849557522123894No Hit
TTTCAATCTTTAAAGTTAAAATCAGGTAGAGCTGCAGTTCCAGCTACTTGC10.8849557522123894No Hit
AAAAACAAAACACAAAAACAAACACAACAAAAAACAAAGAACAAACATCAA10.8849557522123894No Hit
AGTGCAAACAAATTAGAACTCAGGATTAAGAAATTCACTCAAAACTGCACA10.8849557522123894No Hit
TAGGCCTACACAAGTTCAGGATCGATCCACCTACCTTGGTCTTCCAAAGTG10.8849557522123894No Hit
AAAAATCAATGAATCCAGGAGCTGGTTTTGTGAAAGGATCGATCTCCTGCT10.8849557522123894No Hit
TGAACCCAGGAGACGGAGGTTGCAGTGAGCCGAGATCGATCAACTAAAACA10.8849557522123894No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGT10.8849557522123894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGTGGTGGAAGATGGAGTTAGCCTACCAAACTATATCTCCATCCACAA10.8849557522123894No Hit
CACACAAAACACAAAACCACAAACCCCACACCCCCACACAAACCAACCAAA10.8849557522123894No Hit
TGTCCCTGCAGAGATCGATCTATGCCCAAAAGGCCAAACTTCTAGAAGTTC10.8849557522123894No Hit
AAAAACACCAACCAAACCCACAAACCAAAGCAAACACAACCAACAAAAACA10.8849557522123894No Hit
TAATTTTTGTACATGTTGTGATTTAAGGATCGATCTAATATCTGGATTCCA10.8849557522123894No Hit
CCCGTTTCTTCAGCCGTGGGTGCCCCTGGCTCTGCCTCCCCGGGGATCGAT10.8849557522123894No Hit
CCCCCGAGTTCTGGCCAGGAAGCTTCTCGCCCAGTTCAAATTCTTACAAAG10.8849557522123894No Hit
ACCTCAAGTGACTCAGTTTCCTTGGCTATGAAATTCCCATTATGATTCCCT10.8849557522123894No Hit
AAAAAATATTTTGTATTTTATGAAACAATTTACTATTCAACAAAAGATATA10.8849557522123894No Hit
AGCTGCTTGTAACTAAGATGAGAGAGCACTGTGTGCTCCAGCATTTAGATA10.8849557522123894No Hit
GATCACTCTTCCGGAGGTCTCAAGTTCAATTCTCAGCAACCACGTGGTGCC10.8849557522123894No Hit
GAACAACCACCAAACCAAAAACAACACAGAAAACCAAAAACCACAAAAAAA10.8849557522123894No Hit
GGAAAGGAAGGTAAAAGTGGGTACCCACTCTGTACCAGGCATTTTACACAT10.8849557522123894No Hit
CTTTTATACATTTTGCATTTAGTAGCTAACATGTGATGTCAGTACAATTCT10.8849557522123894No Hit
TCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGAGATCTCGGCTCACTG10.8849557522123894No Hit
GTTTCTACCACCACCCCCCATCTGTTGAATTCCAAATCTTTGTCCTTAGCA10.8849557522123894No Hit
TGCCTGCCACACGGGATCGATCAGATTTGGCAGGAGGCACCTTTACCTGCT10.8849557522123894No Hit
CAGCTGTTATTGCTGTCGTTAGTAGCTGGTGATCGATCCACCCAGCTCAGT10.8849557522123894No Hit
GGATGGCAGTTGCATGGACACTCAGCAGCTTAGTCTGTTAAACACACTCTA10.8849557522123894No Hit
AAATAAGTCTTGGGATTTCCAATCAAGATCGATCTGTTTTTAACTTTTTTC10.8849557522123894No Hit
ATCAAGACCCTGTCTCTCCAAAAAAATACAAAAGTTAGCCTGGTGTGGTGG10.8849557522123894No Hit
CTGAATATATTAAAATCAGGATTGTACAACCTACTGAAATTAGAAGCATAA10.8849557522123894No Hit
AGGGTTTTAGGTTGTTATGTTCTGTGCAGTCAGCTGACTCGTATCATCGCC10.8849557522123894No Hit
TATGGATCAAGACTCTTAATAATACATCAAAATTCATTATAAAACACATGA10.8849557522123894No Hit
AACAATTATTATGTACTCGACTGACAATAAAGACATTCTAAAAATTCAAAC10.8849557522123894No Hit
TGGTATCAGCATCACACACAATGCTGAGATGAAAAGTCTTACGCAGGTGTC10.8849557522123894No Hit
CAAAAGGAAACTCATGCAATCATTGTACACAATACAAAGCAATCCTCAACC10.8849557522123894No Hit
ACAGAGTATGTGTGTTTTAAATCTAAACAGTGAAATATACATTTTTGTGTG10.8849557522123894No Hit
AATAAAACAGAAATAAATGCTATTTAGCTGACCAATACTTTTCTTCTGATC10.8849557522123894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)