FastQCFastQC Report
Wed 4 Aug 2021
HGWG2DRXY_n01_MCF7_DMSO_12_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n01_MCF7_DMSO_12_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAAAGACTCAATGTAGTTGACACGTTGATGAGTGCAGCAAACCACCAT21.8691588785046727No Hit
TTATTCTATCAAACATCATTGAGATGCCAGCCTAGAATGGTTATGTTGTGA21.8691588785046727No Hit
TGCTACAGTATTCTTTCTGGTGAAATAGATCGATCAGGGTAGTTAACATAT21.8691588785046727No Hit
ATTACAAAATTAGATACACAGTGGTAAAACTTTGTAAATATACTTAAAGCC21.8691588785046727No Hit
CCTCATTTGTTGGGATCGATCAGCATACCTTTTGTGTCTTCCACCAAATAC10.9345794392523363No Hit
GCACAGCCTGAAGATCGATCATTTAGTTCCCAGATAAAGGACACAAATCTT10.9345794392523363No Hit
TCCCACCAATATCGATCCTTCAGGTCCTTGCGGCTCCTGACAATGAATGAT10.9345794392523363No Hit
AGTTTTTTCAACTACTGTTGTCTGGGCCTCGCTTTTGTAGATTCTGATTCC10.9345794392523363No Hit
TGGCTTCAGCCCTCTCTCTCTCTCCACTGCCTACCACTCTCTCTGCCTTCT10.9345794392523363No Hit
AGGAATAAGGCAGAGGATATACTAAAACATTATTTTTTAAAGTAGTGAATA10.9345794392523363No Hit
GAAAATGTGCATCTGTAAAGAATCTCTGTTAACATAGTTACATCTTTTTCT10.9345794392523363No Hit
GTTGACAAAGGAGATTAACATTTGACTTGTGGGCTGTGAAATGCAGACCCA10.9345794392523363No Hit
ACATTTCCATTCTAGGAAAAAATGGTTAACCATGTCTCTGTTATTGCTTTT10.9345794392523363No Hit
TGATGTTCCAGTCCGTTCAATATGTGTGCAGTTGGAGGAACACATCCTTTC10.9345794392523363No Hit
TAATTTCACATCCCGGAACATTCTCTCTCCAGGCATGATTTTCGTGGCTGC10.9345794392523363No Hit
TTCACTATGAATAATAATAACTATGATCGATCAAGGGAAACCATACATATC10.9345794392523363No Hit
GGCTCCGGCTGCAGAAGAGATGAGGTGCATCTGGCCTTCGTGTGCAGTCGC10.9345794392523363No Hit
GTTAAAATACCCAGTACATAGATGGGATCGATCCTTAAGCTCAATATTATG10.9345794392523363No Hit
TATTTTTGCAAGGTGTGAAAATGGTACTGTGGTTTCTATTTTGAAAAAAAA10.9345794392523363No Hit
CTCTCTCTGTTTATCTGGCTAACTCAAATTTAACCTTTAGGGTTGAGATTA10.9345794392523363No Hit
CGACTCACAGAGTTGAACATTCCTATAGATAGAGCAGGTTGTAAACAATCT10.9345794392523363No Hit
TAACCAAATATGTTCTTCTTAGGTCCATTCATTTTCATTTTATTCTAAAAA10.9345794392523363No Hit
GCTTTTACATTGCAGAATTCAATACTGCACTTCCTACTGGAGATTTATTTT10.9345794392523363No Hit
TCCATTCATTCATGCAACAAATATTAATTGAATGACTCAGCAGTGAACAAA10.9345794392523363No Hit
TAAACAACACCTGGTAAGATCTATCTTAATCATTCTCACTGGTTTAAAGTT10.9345794392523363No Hit
TGCTCTGAGGACCTCACCTCGGTGATCGATCATGTCACTCATCCGTTCAAA10.9345794392523363No Hit
GTGCAGAGATTTGTCAAAATTCCCTGTAGGCAGTGATTATAAAAGTGTTAC10.9345794392523363No Hit
GCCACTAATGATCCTTTGAATTTCTGCAGTATTAATTCTGCAGTTGTAATG10.9345794392523363No Hit
TAGGTATGGGCCTTGAATTCCTGATCGATCATATGCAAGCTGGTCATATCC10.9345794392523363No Hit
TAATCCCAGCACTTGAGATGCTGATCAAAGAGAATTATGAGTTCAAAGTCA10.9345794392523363No Hit
AGAATAACAACCATTCTACCTCATTTGACATTGATGTACCTAATACTCGCT10.9345794392523363No Hit
GATCCAAGTAGGAAGTAATTGCTGTTTAAATATGATGTTGTTAAAGATCGA10.9345794392523363No Hit
GGCTGAGCGAGTGTGTGCGGAGCTGGGGCGGAGGTGGGGAGCACCCGCGGC10.9345794392523363No Hit
CCACGGCCACGGTCACCCGGAAGGCCCTGAGGAACTGCTCTTTGGTGATTT10.9345794392523363No Hit
TTACTCTATGTATTTTGATTATTTTGTAAGTAACCACAGACCAATCCCTCA10.9345794392523363No Hit
TGATGTTCCAGTCCGTTCAATATGTGTGCAGTTGGAGGAACACGTCCTTTC10.9345794392523363No Hit
GCATAGAAACAAGGCTAAAGGTTATTGATCAACATGTTAACAAAATTTCTC10.9345794392523363No Hit
ATTCAGAAAAAGAGACATAGTTTGATCGATCTAAAATGCAAAACACAATTT10.9345794392523363No Hit
CGACATGTCCAAAGACAGTATTTAGTTCTAGTTCACCCCAAGTACTAGCTC10.9345794392523363No Hit
TGCTTTCCCACAATTATCTCAACCACTGCATGGGTTTAACAGCCATGGCCA10.9345794392523363No Hit
GTGGAGCAGGAGGAACAGCCTAGTGGTACCGCTGATCGATCAAGGTGATGA10.9345794392523363No Hit
CGAGGTGAGCTCTGAAAGCAGTAATCCTCAGATCGATCCCAAACACAGAAA10.9345794392523363No Hit
TATGAATATTTTTCCTTACATGACAAAATGTACGTTGAAGATGTGATTTAG10.9345794392523363No Hit
TGGCAAACCAAATTCAGCAGCACATCAAAAAGCTTATCCACCATGATCGAT10.9345794392523363No Hit
AATCTTTTAGACTCTATAAGTTAAGTTCATAAAAACCACTGCTTTCACCCT10.9345794392523363No Hit
TCCCAGCACTTTGAGAGGCCAAGGCAGGGGGATCGATCCCCAATAGTGGGA10.9345794392523363No Hit
TGTGACAAATTATATGGTCAATTTTGGAGAAGATACCATGAGGTGCTGAGA10.9345794392523363No Hit
AGTCCCCTATTAGACACATGATAAAATATTCATTTTTGTCGTTTCAAGATA10.9345794392523363No Hit
GATCCTCCCCAGCCCTACAAAGAGGTTAGGACAGTGACAGGATCGATCTAG10.9345794392523363No Hit
AAGGGGCTGGAGAGATGGCTCGGCTCAGCTGTTAAAAGCACTAATTGCTCT10.9345794392523363No Hit
GCCATCATGCCCGGCTAACTTTTGTATTTTTAGTAGCGATGGGGTTTTCCG10.9345794392523363No Hit
TTTTTTTTTCCAGACAAGAGTGAGGCAGGGTAGAGAAATCACTCACTGAAG10.9345794392523363No Hit
AGAGCCTTTCTCTGATATGAGATCGATCAGAAAACTTAATGAAACACTGCG10.9345794392523363No Hit
TAACATTCTAATAGGCAGATAGGGATAGCACCATCATTATTGACTCCATTG10.9345794392523363No Hit
TCAACTAGAAGATTTAGTATCAGGAACAAACAGACAAATATATACATTTCT10.9345794392523363No Hit
AGCCTCTCTTTTTCCATATCTGAAGCTATATATAAATTTGAATACACATTT10.9345794392523363No Hit
AGTCCTACCCATCAGTAAGGGTATAGCGCAGTGGCATCCCCTCCCCCAACA10.9345794392523363No Hit
TCTGGACTGAGCACGCCACAGCCAGGTAGAAGAGGCTGCGTGTCCTCTTGG10.9345794392523363No Hit
TTAAGAAAAAAGTTCTAAAGATTTTCCATGTTTTAAAATGAAATAATGTTA10.9345794392523363No Hit
CCGCAAAGTATACTTCTGTAGTTACTGCTTTTGAGATTTCTGGATCGATCA10.9345794392523363No Hit
GGGTGGGTGTTGCAGCTTGGTTTGACTTTAGTTTTTGTCACGGGTATGGTT10.9345794392523363No Hit
GGGTGGACCATCATATGTAAAGATCGATCAGGGACAATTTGCCAATATTAT10.9345794392523363No Hit
TGCATGTGTCATTATAGTAGAATGATTTACAATACTTTGGGTATATACCTA10.9345794392523363No Hit
ATATAGCTATTTTGGAATAATAAATAAGAGAAGTAAAAATTTTAAACTCAC10.9345794392523363No Hit
TTTGAACCTGGGACACATATGTTTCAGTGAGCAGAAAACTAACCGCCCTAC10.9345794392523363No Hit
TATTATGAGTCTATCTGCCACAGTAAGGAACAGTCTGCTTCTCATGCCATG10.9345794392523363No Hit
ATATACAGGATACTAATGTAAACCACTAAAAAGTAAAAGACAAATACTTCT10.9345794392523363No Hit
AAATTCTTAATGTTAAACTCTAACCAAAAGACTTGATGAAAAATAACACAT10.9345794392523363No Hit
CCATCAGTGCTAACAAGGTGTAAACATAGTAAAGTGTTCAGAAAAAACCTT10.9345794392523363No Hit
TGTTCTACCTACACTTGAAGGAATTTGAGGGCCACTGGCTTCCTTCAAAGG10.9345794392523363No Hit
GCCGCCTCTTGACCCCCTTTCCGGGGACCCCAGCTCCTCCAGATCGATCCC10.9345794392523363No Hit
TGCTCATAACTATAATCCCAGCACTCTGGGTGGCTGAGATGGGCGGATCGA10.9345794392523363No Hit
TGAATCTAAAAAAAACAGTTTACTAACAGGCGTGAGTGAGGGGCGCTCAGT10.9345794392523363No Hit
CTCAAACGCTTTTTGATCGATCCTTCCTTCCTTCCTTTCTTCCTTCCTTCC10.9345794392523363No Hit
TGCATCTCCATATCTGATGTCAAAGTGGTCTTCAGATCGATCTGTGTACAC10.9345794392523363No Hit
TTCTTTTTCTTTTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTCACCC10.9345794392523363No Hit
AAAAAATATAAAAAAAAACCTGTATCAATGTAATATACCTCTAAACAAAGA10.9345794392523363No Hit
CTGTTCTTTACCTGGAGGTATGTTACAAATTTGAGAGTCATTAGCTTGGAA10.9345794392523363No Hit
GGAAAAGAGCCCCAAGATGGATCGATCTGCAAAGGTAGCAGCTAACCAAGG10.9345794392523363No Hit
TGGTTTTCAGTGTTATCTTGAGTAATCTAAGCCCCTTATCCCAGTGAGGTC10.9345794392523363No Hit
TCTTCAAAATATGAGACAAATTTTGGCTGAGTGATGGTATATGACAAAAAC10.9345794392523363No Hit
TGCTCTGAGGACCTCACCTCTGTGATCGATCATGTCACTCATCCGTTCAAA10.9345794392523363No Hit
AAACCTTGTTGAGTTTTATGAAATAAAGAAAGATCTTCCCACAATGGCATC10.9345794392523363No Hit
TATTACTTGCAATGTCCCTTAGCCTATCTGTGACTCATTTATAAGTTTGTT10.9345794392523363No Hit
AGCAGCGCGGGGCTTGCGGCCAGGGCCGCCTCCCATCCCGTTCCCGAGGTT10.9345794392523363No Hit
ACTTTTATGTGTAGACTAAATGCCGATTGTATATAAATGTACATGTGCGGT10.9345794392523363No Hit
TAGCAGCTGAATTTGTTGTTCCCTTCACCGCTGCAGGACATGTGAAAGTCT10.9345794392523363No Hit
TGGCCAGGCTTGTCTTGAACCCCTGACCTCCAGTGATCGATCCCACCCCAT10.9345794392523363No Hit
CCTTACAAGGATGAAGAATTGGATCGATCTTATCTCAAGATGATAGTGAAG10.9345794392523363No Hit
TGTCCCACACCTTGACTGTCCCATCCCAGGCTACGGGCAAGCTGCTGTCTC10.9345794392523363No Hit
TGGACTCTCTCTCTGTCGCCTAGGCTGGAGTGCAGTGGCCTGATCTCGGCT10.9345794392523363No Hit
GCTCCGAGTCATTTGTGTTGATCGATCTCGACTCACTGCAACCTCCACCTC10.9345794392523363No Hit
GATGGGAAGGCCACTGATGAGGGAAGAAGAACAGAGGAGGTGGCGTGGCAC10.9345794392523363No Hit
TAGCTACTCAGGGAGCTTGAGGCAGTGGGGCCTTGTGAACCCAGGAGTTTG10.9345794392523363No Hit
GACTTCAACACACCACTTTCACCAATGGACAGATCATGGAAACAGAAACTA10.9345794392523363No Hit
CAAAGGAGAGCATGGAAGGGAAAAAATGGATGGCTGCATGTTGTCAAACCG10.9345794392523363No Hit
TGATCTTCCTGACTCAGCCTCCTGAATAGCTAAGACTACAGGCACACACCG10.9345794392523363No Hit
AGACATCAATGGATAACAAGATTGGATTCGCCCCTGCCACTTTAAGAACTC10.9345794392523363No Hit
TGATATTTTCCCAGTAACTTTTCTCCTCCTTCTTGACTCCATCTTTTCCAT10.9345794392523363No Hit
TATATTCCTTCAATGCCTAGTTTCTTGAGGGTTTTTATCATAAATGGATGT10.9345794392523363No Hit
TGATTGCACCCAAGTATTTCCCTCCATCAGTGAGGCAGTACAGTGGGGCCT10.9345794392523363No Hit
TCATTAGTACAGAATTCAAGAATGTTGAATTGGTGTGGTTAACACAATACA10.9345794392523363No Hit
GTGTTAGCTTCTGGTGAACATCTGGAGCTGGCCACTCATGCTCTGCAGTCT10.9345794392523363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)