FastQCFastQC Report
Wed 11 Aug 2021
HGWG2DRXY_n01_HCT116_Vorino_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWG2DRXY_n01_HCT116_Vorino_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76587214
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT2617290.34173981051197394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1726430.22542013344420653Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC966580.12620644485122542Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC820870.10718107594304188Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)