FastQCFastQC Report
Wed 30 Jun 2021
HGW2MDRXY_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGW2MDRXY_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990389104
Sequences flagged as poor quality0
Sequence length90
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG144383160.7254016800526054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA57563230.2892059139809278Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCATATCAATAAGCGGAGGA44487100.2235095635853119Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC39672580.19932072538114137Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATCAATAAGCGGAGGAAA27299910.13715865880262576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG25887060.1300602979988982Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG24937210.12528811552416938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22338560.11223212564371032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)