FastQCFastQC Report
Wed 30 Jun 2021
HGW2MDRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGW2MDRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990389104
Sequences flagged as poor quality0
Sequence length10
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACACTAG56128988928.20000812263289No Hit
GACTCTCCCG53828520427.044219791910596No Hit
GCATTACGTA39736099519.963985644889263No Hit
TTCACGCATA34624166417.39567722231663No Hit
AGATCTCGGT664004703.3360547375665295Illumina Single End PCR Primer 1 (100% over 10bp)
CAGGGTTGGC126140910.6337500026828925No Hit
GATGAAGATA111630880.5608495332679434No Hit
GAAGTTAGGG102050340.5127155278076724No Hit
CTCCAAGTTC71994260.361709476078402No Hit
GGGGGGGGGG54520740.27392000835631586No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)