FastQCFastQC Report
Wed 30 Jun 2021
HGW2MDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGW2MDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990389104
Sequences flagged as poor quality0
Sequence length10
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGACGAAA56506223728.389536290387568No Hit
TCTACCATTT53503131126.880739546090282No Hit
AATGCCATGA39410536119.800417928734802No Hit
GCACTGAGAA32342143816.249156376008777No Hit
GGGGGGGGGG787697643.9575057882752556No Hit
GTGGATCAAA121361010.6097351003183547No Hit
CTCTAGCGAG111726930.5613321022279872No Hit
TAACGCGTGA102090410.5129168452280676No Hit
CGAAGTATAC71168060.35755852891767037No Hit
TCTACCATTA35889420.1803135875687551No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)