FastQCFastQC Report
Mon 23 Aug 2021
HGVMYDRXY_n02_CIVRCoV_P2_B08_Neg2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVMYDRXY_n02_CIVRCoV_P2_B08_Neg2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28201
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATAGGCTGTGTAGATC6812.414807985532428Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3281.1630793234282473No Hit
ATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTAT1070.3794191695329953No Hit
AATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAA690.24467217474557643No Hit
ATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAAT630.2233963334633524No Hit
TAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATA560.19857451863409098No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG510.1808446508989043Illumina Single End PCR Primer 1 (100% over 32bp)
TATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTA430.15247686252260556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG430.15247686252260556No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG420.14893088897556825Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT370.13120102124038155No Hit
ATAATTATTATTATAATTATAATTATAATTATAATTATAATTATAATTAT370.13120102124038155No Hit
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATAGGCT320.11347115350519485Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG300.10637920641112017Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)