FastQCFastQC Report
Mon 23 Aug 2021
HGVMYDRXY_n02_CIVRCoV_P1_E04_Neg1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVMYDRXY_n02_CIVRCoV_P1_E04_Neg1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21165
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGATC5322.5135837467517126Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5132.4238128986534373Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT4572.1592251358374672Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4512.130876446964328No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2451.1575714623198678Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG2110.9969288920387432Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1500.7087172218284904Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1120.5291755256319395Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG960.45357902197023386Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT720.3401842664776754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG590.2787621072525395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG480.22678951098511693No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA470.22206472950626033Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG430.20316560359083394Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG410.19371604063312073Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT350.1653673517599811No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG340.1606425702811245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT340.1606425702811245No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG320.1511930073234113Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG310.1464682258445547Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC300.14174344436569808No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT270.1275690999291283No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAA260.12284431845027168Illumina Single End PCR Primer 1 (96% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTG260.12284431845027168Illumina Single End PCR Primer 1 (96% over 32bp)
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTG250.11811953697141507Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG240.11339475549255847No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG220.10394519253484527Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT220.10394519253484527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)