FastQCFastQC Report
Mon 23 Aug 2021
HGVMYDRXY_n01_CIVRCoV_P2_F04_C003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVMYDRXY_n01_CIVRCoV_P2_F04_C003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33139787
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC2853920.8611763256052309No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA2774890.8373288579072642No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2408340.7267216292005739No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1344970.40584750891730237No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1292850.39012018996983894No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG1223570.36921480515248933No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1207750.36444108708363154No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT1054790.31828508734832844No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1047630.31612454238163934No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC1044400.31514988312990666No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC980830.2959675027482826No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA978590.29529157806596645No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG873030.2634386274118177No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC841630.25396361177577875No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC830900.25072581184664827No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC821680.24794365757390052No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC814140.2456684468128899No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC774080.23358025807468227No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA743890.22447036246793015No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA738000.22269304265594708No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC737870.22265381488420552No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC725180.21882458085804835No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA712090.21487464599576336No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC704310.21252701473307598No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC703550.212297683144433No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA685990.20699891643841886No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG678280.20467240782205387No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA675620.20386974726180346No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC673110.203112349515101No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT654290.197433375175284No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG651470.19658243428058242No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT644520.19448525725286042No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC636500.19206520548849634No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT620290.18717380410441384No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT609810.18401144219786325No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG584890.17649178010709604No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC583930.17620209810038912No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT580470.17515803586788292No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC576280.17389369460944332No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG566380.1709063489152782No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC562110.16961786748961302No Hit
TCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAG545260.16453334476772585No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA543600.16403243629779515No Hit
GGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACG542860.16380913975095857No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC540800.16318753044489998No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG540750.16317244284038399No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG533930.16111449358440355No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC530070.15994973051576947No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA522860.15777409794456432No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC516780.15593944523542047No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA515020.1554083615564578No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG509230.15366121695350668No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT504100.15211322873016656No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA498940.15055618794411685No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG483940.14602990658932116No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT482470.1455863310165512No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT482350.14555012076571283No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC481140.14518500073642598No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG479530.14469917987101125No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG472500.14257786267606365No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG464960.14030265191505303No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA461020.13911374867919338No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT460970.1390986610746774No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA460540.13896890767583991No Hit
GAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCA456230.13766835616656198No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA449350.13559230178516235No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC447310.13497672752091014No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG445750.1345059942600114No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA438170.13221871341538796No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT437820.1321131001837761No Hit
CCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCG433200.130719005526499No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG428660.1293490510364475No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG417670.1260327955638339No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA416530.12568879818086942No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG415880.1254926593221616No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG415280.1253116080679698No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG408690.12332306179276288No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT408140.12315709814308703No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC402630.12149444412542541No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC397360.11990421060944056No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG394010.11889334110686953No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG391550.11815103096468303No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC390980.1179790322732008No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG389690.11758977207668836No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA389420.11750829901230204No Hit
CTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCC385700.11638578123631271No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT385470.11631637825553919No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA382480.11541413950548324No Hit
CGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCC381210.1150309143507772No Hit
CAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGC381050.11498263401632605No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA380550.1148317579711662No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA377270.11384201111491754No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA375900.11342861075117955No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG372110.11228497032886783No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT371980.11224574255712629No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG369450.11148230976861741No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT364000.10983776087637497No Hit
CAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACC363870.10979853310463342No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC358710.10824149231858371No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC357390.10784317955936168No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC356460.10756255011536435No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG355930.1074026215074949No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG355830.10737244629846292No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC355010.10712500958440076No Hit
GGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCG354990.10711897454259438No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA353850.1067749771596299No Hit
CCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAA352630.10640683960943986No Hit
TGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAG350000.10561323161189902No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA345610.10428853993539547No Hit
GGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGG345160.1041527514947516No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA344850.10405920834675249No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG344750.10402903313772054No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT344380.10391738486430224No Hit
CGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC341490.10304532132327827No Hit
CGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCG338370.10210385480148078No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG338130.10203143429980403No Hit
CGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGT337260.1017689099812259No Hit
GTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCG336230.10145810532819659No Hit
GCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAG332960.10047137599285114No Hit
TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTC332330.1002812721759497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)